Jung Yong-Taek, Park Sooyeon, Lee Jung-Sook, Yoon Jung-Hoon
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4191-4197. doi: 10.1099/ijs.0.068767-0. Epub 2014 Sep 24.
A Gram-stain-negative, coccoid- or short-rod-shaped and non-gliding bacterial strain, designated CDM-7(T), was isolated from the zone where the ocean meets a freshwater spring at Jeju island, South Korea, and was subjected to a polyphasic taxonomic study. Strain CDM-7(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain CDM-7(T) falls within the clade comprising species of the genus Defluviimonas, clustering with the type strain of Defluviimonas aestuarii, with which it exhibited the highest 16S rRNA gene sequence similarity value (98.4 %). The 16S rRNA gene sequence similarity values between strain CDM-7(T) and the type strains of Defluviimonas denitrificans and Defluviimonas indica were 97.1 and 96.2 %, respectively. The genomic DNA G+C content was 66.8 mol% and the mean DNA-DNA relatedness values between strain CDM-7(T) and the type strains of D. aestuarii and D. denitrificans were 15.6±2.5 and 6.7±3.2 %, respectively. Strain CDM-7(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain CDM-7(T) is distinguishable from other species of the genus Defluviimonas. On the basis of the data presented, strain CDM-7(T) is considered to represent a novel species of the genus Defluviimonas, for which the name Defluviimonas aquaemixtae sp. nov. is proposed. The type strain is CDM-7(T) ( = KCTC 42108(T) = CECT 8626(T)).
从韩国济州岛海洋与淡水泉交汇处分离出一株革兰氏染色阴性、球状或短杆状且不滑动的细菌菌株，命名为CDM-7(T)，并对其进行了多相分类研究。菌株CDM-7(T)在pH 7.0-8.0、30°C以及2-3%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法、最大似然法和最大简约法系统发育树表明，菌株CDM-7(T)属于包含脱流单胞菌属物种的进化枝，与河口脱流单胞菌模式菌株聚类，与其16S rRNA基因序列相似性最高（98.4%）。菌株CDM-7(T)与反硝化脱流单胞菌和印度脱流单胞菌模式菌株的16S rRNA基因序列相似性分别为97.1%和96.2%。基因组DNA G+C含量为66.8 mol%，菌株CDM-7(T)与河口脱流单胞菌和反硝化脱流单胞菌模式菌株的平均DNA-DNA相关性值分别为15.6±2.5%和6.7±3.2%。菌株CDM-7(T)以Q-10作为主要泛醌，以总和特征8（C18:1ω7c和/或C18:1ω6c）作为主要脂肪酸。主要极性脂质为磷脂酰胆碱、磷脂酰甘油、一种未鉴定的氨基脂质、一种未鉴定的磷脂和一种未鉴定的脂质。不同的表型特征，以及系统发育和遗传独特性，表明菌株CDM-7(T)与脱流单胞菌属的其他物种不同。根据所提供的数据，菌株CDM-7(T)被认为代表脱流单胞菌属的一个新物种，为此提出新名称混合水脱流单胞菌（Defluviimonas aquaemixtae sp. nov.）。模式菌株为CDM-7(T)（=KCTC 42108(T)=CECT 8626(T)）。
