Park Sooyeon, Choi Jeehyun, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Curr Microbiol. 2018 Nov;75(11):1516-1522. doi: 10.1007/s00284-018-1553-6. Epub 2018 Aug 20.
A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57 grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57 exhibited the highest 16S rRNA gene sequence similarity value (94.2%) to the type strain of Pontivivens insulae forming a cluster in the neighbour-joining phylogenetic tree. In the maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences and the phylogenetic tress based on gyrB sequences, strain OITF-57 formed evolutionary lineages independent of those of other taxa. Strain OITF-57 contained Q-10 as the predominant ubiquinone and C ω7c as the major fatty acid. The major polar lipids of strain OITF-57 were phosphatidylcholine and phosphatidylglycerol. The DNA G + C content of strain OITF-57 was 66.0 mol%. The chemotaxonomic data and other differential phenotypic properties made it possible to distinguish strain OITF-57 from the genus Pontivivens and other phylogenetically related genera. On the basis of the data presented, strain OITF-57 constitutes a new genus and species within the class Alphaproteobacteria, for which the name Pseudopontivivens aestuariicola gen. nov., sp. nov. is proposed. The type strain is OITF-57 (= KACC 19570 = CGMCC 1.13481).
从韩国潮滩沉积物中分离出的革兰氏阴性、无运动性、球状、卵形或杆状细菌菌株OITF-57，进行了分类学特征分析。菌株OITF-57在25℃、pH 7.0 - 8.0以及2.0%（w/v）NaCl存在的条件下生长最佳。菌株OITF-57与Pontivivens insulae模式菌株的16S rRNA基因序列相似性最高（94.2%），在邻接法系统发育树中形成一个簇。在基于16S rRNA基因序列的最大似然法和最大简约法系统发育树以及基于gyrB序列的系统发育树中，菌株OITF-57形成了独立于其他分类单元的进化分支。菌株OITF-57以Q-10作为主要泛醌，以C ω7c作为主要脂肪酸。菌株OITF-57的主要极性脂类为磷脂酰胆碱和磷脂酰甘油。菌株OITF-57的DNA G + C含量为66.0 mol%。化学分类学数据和其他差异表型特征使得菌株OITF-57能够与Pontivivens属及其他系统发育相关属区分开来。基于所呈现的数据，菌株OITF-57在α-变形菌纲内构成一个新属和新种，为此提出新属名Pseudopontivivens aestuariicola gen. nov., sp. nov.。模式菌株为OITF-57（= KACC 19570 = CGMCC 1.13481）。
