Park Sooyeon, Jung Yong-Taek, Choi Su Jung, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3446-3451. doi: 10.1099/ijsem.0.002136. Epub 2017 Aug 25.
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated JSSK-8T, was isolated from the place where the ocean and a freshwater spring meet at Jeju Island, South Korea. Strain JSSK-8T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2.0 % (w/v) NaCl. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain JSSK-8T fell within the clade comprising the type strains of species of the genus Altererythrobacter. Strain JSSK-8T exhibited 16S rRNA gene sequence similarity values of 97.7 and 97.2 % to the type strains of Altererythrobacteraestiaquae and Altererythrobacterluteolus, respectively, and of 93.5-96.2 % to the type strains of the other species of the genus Altererythrobacter. Strain JSSK-8T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C17 : 1ω6c, C16 : 0 and 11-methyl C18 : 1ω7c as the major fatty acids. The major polar lipids detected in strain JSSK-8T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain JSSK-8T was 59.0 mol%, and its mean DNA-DNA relatedness values with the type strains of A. aestiaquae and A. luteolus were 13 and 16 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain JSSK-8T is separated from recognized species of the genus Altererythrobacter. On the basis of the data presented, strain JSSK-8T is considered to represent a novel species of the genus Altererythrobacter, for which the name Altererythrobacter aquaemixtae sp. nov. is proposed. The type strain is JSSK-8T (=KCTC 52763T=NBRC 112764T).
从韩国济州岛海洋与淡水泉交汇处分离出一株革兰氏染色阴性、需氧、无运动性、卵圆形或杆状的细菌菌株，命名为JSSK-8T。菌株JSSK-8T在pH 7.0-8.0、30 °C以及2.0%（w/v）NaCl存在的条件下生长最佳。在基于16S rRNA基因序列构建的邻接法系统发育树中，菌株JSSK-8T位于包含交替赤杆菌属各物种模式菌株的进化枝内。菌株JSSK-8T与嗜盐交替赤杆菌和浅黄交替赤杆菌模式菌株的16S rRNA基因序列相似性值分别为97.7%和97.2%，与交替赤杆菌属其他物种模式菌株的相似性值为93.5%-96.2%。菌株JSSK-8T以Q-10作为主要泛醌，以C18 : 1ω7c、总和特征3（C16 : 1ω6c和/或C16 : 1ω7c）、C17 : 1ω6c、C16 : 0和11-甲基C18 : 1ω7c作为主要脂肪酸。在菌株JSSK-8T中检测到的主要极性脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和鞘糖脂。菌株JSSK-8T的DNA G+C含量为59.0 mol%，其与嗜盐交替赤杆菌和浅黄交替赤杆菌模式菌株的平均DNA-DNA相关性值分别为13%和16%。不同的表型特征，以及系统发育和遗传独特性，表明菌株JSSK-8T与已确认的交替赤杆菌属物种不同。基于所提供的数据，菌株JSSK-8T被认为代表交替赤杆菌属的一个新物种，为此提出名为混水交替赤杆菌（Altererythrobacter aquaemixtae）的新种名。模式菌株为JSSK-8T（=KCTC 52763T=NBRC 112764T）。
