Chen Huan-Yuan, Weng I-Chun, Li Chi-Shan, Wan Lei, Liu Fu-Tong
Department of Dermatology, University of California Davis, School of Medicine, Sacramento, CA, USA.
Methods Mol Biol. 2015;1207:201-13. doi: 10.1007/978-1-4939-1396-1_13.
Galectins, a family of β-galactoside-binding proteins, are expressed in many different phagocytic leukocytes (granulocytes, monocytes, and macrophages). A number of family members have been shown to play an important role in ingestion of particles (phagocytosis), thus contributing to clearance of damaged cells and host defense against pathogens. Here we describe procedures for analysis of the roles of galectins in phagocytosis by using galectin-3 as an example. We emphasize the function of endogenous galectin-3 as determined by comparison of phagocytosis by macrophages from galectin-3 knockout mice and wild-type mice. We focus on the role of galectin-3 in phagocytosis of pathogens and Fcγ receptor-mediated phagocytosis of opsonized cells and particles.
半乳糖凝集素是一类β-半乳糖苷结合蛋白，在许多不同的吞噬性白细胞（粒细胞、单核细胞和巨噬细胞）中表达。已表明许多家族成员在颗粒摄取（吞噬作用）中起重要作用，从而有助于清除受损细胞和宿主抵御病原体。在此，我们以半乳糖凝集素-3为例，描述分析半乳糖凝集素在吞噬作用中作用的方法。我们强调通过比较来自半乳糖凝集素-3基因敲除小鼠和野生型小鼠的巨噬细胞的吞噬作用来确定内源性半乳糖凝集素-3的功能。我们重点关注半乳糖凝集素-3在病原体吞噬作用以及Fcγ受体介导的调理素化细胞和颗粒的吞噬作用中的作用。
