Mendki Murlidhar J, Ganesan K, Parashar B D, Sukumaran D, Prakash Shri
Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
J Vector Borne Dis. 2014 Sep;51(3):224-9.
BACKGROUND & OBJECTIVES: Bedbug Cimex hemipterus lives in cracks and crevices, and shows aggregation activity in the harbourage containing its fecal matter. Limited information is available on the chemicals influencing this aggregation behaviour in C. hemipterus. Possible components responsible for aggregation, if identified and evaluated can be helpful in designing bedbug management.
Study on the isolation and identification of chemicals from the excreta extract responsible for aggregation has been carried by GC-MS. The compounds identified were evaluated at different concentrations to C. hemipterus and their attraction index was determined.
The GC-MS analysis of the excreta extract resulted in identification of 33 different types of semiochemicals such as esters, carboxylic acids, alcohols, aldehydes, ketones and other hydrocarbons. Among these 33 compounds of various groups, the carboxylic acid (E)-2-hexenoic acid and the aldehyde (E)-2-hexenal found to elicit highest positive aggregation behavioural response in all the stages followed by hexenal.
INTERPRETATION & CONCLUSION: The fecal matter influence the aggregation behaviour in C. hemipterus. (E)-2-hexenoic acid, hexanoic acid, (E)-2-hexenal and hexanal found to exhibit aggregation in various stages of bedbug life cycle. These chemical components can be further exploited for designing and development tool for management of bedbugs.
臭虫热带臭虫生活在裂缝和缝隙中，在含有其粪便的栖息场所表现出聚集活动。关于影响热带臭虫这种聚集行为的化学物质的信息有限。如果能够识别并评估可能导致聚集的成分，将有助于设计臭虫管理方法。
采用气相色谱 - 质谱联用（GC-MS）对负责聚集的排泄物提取物中的化学物质进行分离和鉴定研究。对鉴定出的化合物以不同浓度作用于热带臭虫，并测定其吸引指数。
排泄物提取物的GC-MS分析鉴定出33种不同类型的信息素，如酯类、羧酸类、醇类、醛类、酮类和其他烃类。在这33种不同类别的化合物中，羧酸（E）-2-己烯酸和醛（E）-2-己烯醛在所有发育阶段均引发最高的正向聚集行为反应，其次是己醛。
粪便影响热带臭虫的聚集行为。（E）-2-己烯酸、己酸、（E）-2-己烯醛和己醛在臭虫生命周期的各个阶段均表现出聚集作用。这些化学成分可进一步用于设计和开发臭虫管理工具。
