Popova G Iu, Eremeev N L, Aĭsina R B, Kazanskaia N F
Biull Eksp Biol Med. 1989 May;107(5):561-4.
A simple and sensitive method has been developed to assess the fibrinolytic activity of plasmin from the change in the column height of fibrin gel. Two conditions were used: 1) 37 degrees C and 16 h incubation at plasmin concentrations of 0.5-50 micrograms/ml and 2) 25 degrees C and 1-2.5 h incubation at plasmin concentrations of 50-1000 micrograms/ml. The method permits to observe the kinetics of fibrinolysis at plasmin concentrations higher that 10 micrograms/ml. The results have shown that the method is applicable for quantitation of plasminogen in human plasma. The method is precise and well reproducible.
已开发出一种简单而灵敏的方法，可根据纤维蛋白凝胶柱高度的变化来评估纤溶酶的纤溶活性。使用了两种条件：1）在37℃下，纤溶酶浓度为0.5 - 50微克/毫升时孵育16小时；2）在25℃下，纤溶酶浓度为50 - 1000微克/毫升时孵育1 - 2.5小时。该方法能够观察纤溶酶浓度高于10微克/毫升时的纤溶动力学。结果表明，该方法适用于定量人血浆中的纤溶酶原。该方法精确且可重复性良好。
