Lü Zhi-Chuang, Li Qian, Liu Wan-Xue, Wan Fang-Hao
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China; Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing, China.
PLoS One. 2014 Sep 25;9(9):e108428. doi: 10.1371/journal.pone.0108428. eCollection 2014.
Temperature is an important factor in affecting population dynamics and diffusion distribution of organisms. Alien species can successfully invade and colonize to various temperature environments, and one of important reasons is that alien species have a strong resistance to stress temperature. Recently, researchers have focused on the mechanisms of temperature sensing to determine the sensing and regulation mechanisms of temperature adaptation. The transient receptor potential (TRP) is one of the key components of an organism's temperature perception system. TRP plays important roles in perceiving temperature, such as avoiding high temperature, low temperature and choosing the optimum temperature. To assess high temperature sensation and the heat resistance role of the TRP gene, we used 3' and 5' rapid-amplification of cDNA ends to isolate the full-length cDNA sequence of the TRP gene from Bemisia tabaci (Gennadius) MEAM1 (Middle East Asia Minor 1), examined the mRNA expression profile under various temperature conditions, and identified the heat tolerance function. This is the first study to characterize the TRP gene of invasive B. tabaci MEAM1 (MEAM1 BtTRP). The full-length cDNA of MEAM1 BtTRP was 3871 bp, and the open reading frames of BtTRP was 3501 bp, encoding 1166 amino acids. Additionally, the BtTRP mRNA expression level was significantly increased at 35°C. Furthermore, compared with control treatments, the survival rate of B. tabaci MEAM1 adults was significantly decreased under high temperature stress conditions after feeding with dsRNA BtTRP. Collectively, these results showed that MEAM1 BtTRP is a key element in sensing high temperature and plays an essential role in B. tabaci MEAM1 heat tolerance ability. Our data improved our understanding of the mechanism of temperature sensation in B. tabaci MEAM1 at the molecular level and could contribute to the understanding of the thermal biology of B. tabaci MEAM1 within the context of global climate change.
温度是影响生物种群动态和扩散分布的重要因素。外来物种能够成功入侵并定殖于各种温度环境，其中一个重要原因是外来物种对胁迫温度具有较强的抗性。近年来，研究人员聚焦于温度感知机制，以确定温度适应的感知和调节机制。瞬时受体电位（TRP）是生物体温度感知系统的关键组成部分之一。TRP在感知温度方面发挥着重要作用，如避免高温、低温以及选择最适温度。为评估TRP基因的高温感知和耐热作用，我们利用3'和5' cDNA末端快速扩增技术从烟粉虱（Gennadius）MEAM1（中东-小亚细亚1型）中分离出TRP基因的全长cDNA序列，检测了其在不同温度条件下的mRNA表达谱，并鉴定了耐热功能。这是首次对入侵性烟粉虱MEAM1（MEAM1 BtTRP）的TRP基因进行表征的研究。MEAM1 BtTRP的全长cDNA为3871 bp，BtTRP的开放阅读框为3501 bp，编码1166个氨基酸。此外，BtTRP mRNA表达水平在35°C时显著升高。进一步研究发现，与对照处理相比，用dsRNA BtTRP饲喂后，烟粉虱MEAM1成虫在高温胁迫条件下的存活率显著降低。综上所述，这些结果表明MEAM1 BtTRP是感知高温的关键元件，在烟粉虱MEAM1的耐热能力中发挥着重要作用。我们的数据在分子水平上增进了我们对烟粉虱MEAM1温度感知机制的理解，并有助于在全球气候变化背景下理解烟粉虱MEAM1的热生物学特性。
