Tehseen Muhammad, Liao Chunyan, Dacres Helen, Dumancic Mira, Trowell Stephen, Anderson Alisha
CSIRO Food Futures National Research Flagship & CSIRO Ecosystem Sciences, Australia, Canberra, ACT, Australia; Center for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
CSIRO Food Futures National Research Flagship & CSIRO Ecosystem Sciences, Australia, Canberra, ACT, Australia.
PLoS One. 2014 Sep 25;9(9):e108680. doi: 10.1371/journal.pone.0108680. eCollection 2014.
It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
脊椎动物的G蛋白偶联受体（GPCRs）以同二聚体、异二聚体或更高阶寡聚体的形式相互关联，这一点已被广泛接受。秀丽隐杆线虫基因组编码数百种嗅觉GPCR，它们可能在不到一打化学感受神经元中表达，这表明存在寡聚化的机会。在这里，我们使用三种独立的证据：免疫共沉淀、生物发光共振能量转移和酵母双杂交试验表明，线虫嗅觉受体（ORs）在酵母中异源表达时会发生寡聚化。具体而言，线虫受体ODR-10能够同寡聚化，也能与相关的线虫受体STR-112形成异聚体。ODR-10还能与大鼠I7 OR寡聚化，但不能与人促生长激素抑制素受体5（一种神经肽受体）寡聚化。在本研究中，未涉及功能相关性问题，仍有待研究。
