Sengupta P, Chou J H, Bargmann C I
Howard Hughes Medical Institute, Programs in Developmental Biology, Neuroscience, and Genetics, University of California, San Francisco, 94143-0452, USA.
Cell. 1996 Mar 22;84(6):899-909. doi: 10.1016/s0092-8674(00)81068-5.
Olfactory signaling is initiated by interactions between odorants and olfactory receptors. We show that the C. elegans odr-10 gene is likely to encode a receptor for the odorant diacetyl. odr-10 mutants have a specific defect in chemotaxis to diacetyl, one of several odorants detected by the AWA olfactory neurons. odr-10 encodes a predicted seven transmembrane domain receptor; a green fluorescent protein-tagged Odr-10 protein is localized to the AWA sensory cilia. odr-10 expression is regulated by odr-7, a transcription factor implicated in AWA sensory specification. Expression of odr-10 from a heterologous promoter directs behavioral responses to diacetyl, but not to another odorant detected by the AWA neurons. These results provide functional evidence for a specific interaction between an olfactory receptor protein and its odorant ligand.
嗅觉信号传导由气味分子与嗅觉受体之间的相互作用引发。我们发现秀丽隐杆线虫的odr - 10基因可能编码气味分子双乙酰的受体。odr - 10突变体在对双乙酰的趋化性上存在特定缺陷，双乙酰是AWA嗅觉神经元检测到的几种气味分子之一。odr - 10编码一种预测的七跨膜结构域受体；绿色荧光蛋白标记的Odr - 10蛋白定位于AWA感觉纤毛。odr - 10的表达受odr - 7调控，odr - 7是一种与AWA感觉特异性有关的转录因子。从异源启动子表达odr - 10可引发对双乙酰的行为反应，但对AWA神经元检测到的另一种气味分子则无此反应。这些结果为嗅觉受体蛋白与其气味分子配体之间的特异性相互作用提供了功能证据。
