Department of Medical System Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Republic of Korea.
Korea Basic Science Institute, Daejeon 169-148, Republic of Korea.
Biosens Bioelectron. 2015 Feb 15;64:338-44. doi: 10.1016/j.bios.2014.09.025. Epub 2014 Sep 16.
Norovirus (NoV) is a foodborne pathogen that can cause sporadic and epidemic gastrointestinal diseases. Rapid screening is crucial to promptly identify the presence of NoV and prevent food poisoning. Here, we present a sensitive, selective, and rapid electrochemical biosensor for the detection of NoV. The proposed electrochemical biosensor is composed of a nanostructured gold electrode conjugated with concanavalin A (ConA). ConA functions as a recognition element that selectively captures NoV. Cyclic voltammetry revealed a linear relationship (R(2) = 0.998) between the current and concentration of NoV (in the range of 10(2) and 10(6) copies/mL), with a relatively short assay time (1h) and a good detection limit (35 copies/mL). Additionally, the signals of Hepatitis A and E in the selectively test were found to be only 2.0% and 2.8% of the NoV signal at an identical concentration of 10(3) copies/mL, proving that the electrochemical biosensor has a selectively of approximately 98%. Moreover, the concentration of NoV was measured in a realistic environment, i.e., a sample solution extracted from lettuce, to demonstrate a potential application of the proposed biosensor (LoD = 60 copies/mL).
诺如病毒（NoV）是一种食源性病原体，可导致散发性和流行性胃肠道疾病。快速筛查对于及时发现 NoV 并预防食物中毒至关重要。在这里，我们提出了一种用于检测 NoV 的敏感、选择性和快速电化学生物传感器。所提出的电化学生物传感器由与伴刀豆球蛋白 A（ConA）偶联的纳米结构金电极组成。ConA 作为识别元件，可选择性地捕获 NoV。循环伏安法显示 NoV 的电流与浓度之间存在线性关系（R² = 0.998）（在 10²和 10⁶拷贝/mL 的范围内），具有相对较短的测定时间（1 小时）和良好的检测限（35 拷贝/mL）。此外，在相同浓度为 10³拷贝/mL 时，选择性测试中甲型肝炎和戊型肝炎的信号仅为 NoV 信号的 2.0%和 2.8%，证明电化学生物传感器的选择性约为 98%。此外，还在实际环境中（即从生菜中提取的样品溶液）测量了 NoV 的浓度，以证明所提出的生物传感器的潜在应用（LoD = 60 拷贝/mL）。
