Lee Inae, So Heejin, Ho Kacie K H Y, Li Yong, Jun Soojin
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Department of Human Nutrition, Food, and Animal Sciences, University of Hawaii, Honolulu, HI 96822, USA.
Biosensors (Basel). 2025 Jun 3;15(6):353. doi: 10.3390/bios15060353.
Norovirus, a foodborne pathogen, causes a significant economic and health burden globally. Although detection methods exist, they are expensive and non-field deployable. A flow-based dielectrophoretic biosensor was designed for the detection of foodborne pathogenic viruses and was tested using bacteriophage MS2 as a norovirus surrogate. The flow-based MS2 sensor comprises a concentrator and a detector. The concentrator is an interdigitated electrode array designed to impart dielectrophoretic effects to manipulate viral particles toward the detector in a fluidic channel. The detector is made of a silver electrode conjugated with anti-MS2 IgG to allow for antibody-antigen biorecognition events and is supplied with the electrical current for the purpose of measurement. Serially diluted MS2 suspensions were continuously injected into the fluidic channel at 0.1 mL/min. A cyclic voltammogram indicated that current measurements from single-walled carbon nanotube (SWCNT)-coated electrodes increased compared to uncoated electrodes. Additionally, a drop in the current measurements after antibody immobilization and MS2 capture was observed with the developed electrodes. Antibody immobilization at the biorecognition site provided greater current changes with the antibody-MS2 complexes vs. the assays without antibodies. The electric field applied to the fluidic channel at 10 V and 1 MHz contributed to an increase in current changes in response to MS2 bound on the detector and was dependent on the MS2 concentrations in the sample. The developed biosensor was able to detect MS2 with a sensitivity of 10 PFU/mL within 15 min. Overall, this work demonstrates a proof of concept for a rapid and field-deployable strategy to detect foodborne pathogens.
诺如病毒是一种食源性病原体，在全球范围内造成了重大的经济和健康负担。尽管存在检测方法，但它们价格昂贵且无法在现场部署。设计了一种基于流动的介电泳生物传感器用于检测食源性病原体，并使用噬菌体MS2作为诺如病毒替代物进行了测试。基于流动的MS2传感器包括一个浓缩器和一个检测器。浓缩器是一个叉指电极阵列，设计用于施加介电泳效应，以在流体通道中将病毒颗粒操纵向检测器。检测器由与抗MS2 IgG共轭的银电极制成，以允许抗体-抗原生物识别事件发生，并提供电流用于测量。将系列稀释的MS2悬浮液以0.1 mL/min的流速连续注入流体通道。循环伏安图表明，与未涂层电极相比，单壁碳纳米管（SWCNT）涂层电极的电流测量值增加。此外，在使用已开发的电极时，观察到抗体固定和MS2捕获后电流测量值下降。在生物识别位点固定抗体后，与无抗体的检测相比，抗体-MS2复合物产生了更大的电流变化。在10 V和1 MHz下施加到流体通道的电场导致响应于结合在检测器上的MS2的电流变化增加，并且取决于样品中MS2的浓度。所开发的生物传感器能够在15分钟内以10 PFU/mL的灵敏度检测MS2。总体而言，这项工作证明了一种用于检测食源性病原体的快速且可现场部署策略的概念验证。
