心脏中免疫反应性心房利钠肽的系统发育研究。

Phylogenetic study on the immunoreactive atrial natriuretic peptide in the heart.

作者信息

Kim S H, Cho K W, Koh G Y, Seul K H, So J N, Ryu H

机构信息

Department of Physiology, Jeonbug National University Medical School, Jeonju, Republic of Korea.

出版信息

Gen Comp Endocrinol. 1989 Apr;74(1):127-35. doi: 10.1016/0016-6480(89)90121-4.

DOI:10.1016/0016-6480(89)90121-4

PMID:2525503

Abstract

Using two antisera against atriopeptin III (AP III) which had different characteristics in cross-reactivities with atrial natriuretic peptide (ANP) analogs, we have measured an immunoreactive ANP (ir-ANP) in the heart extracts of several species. ir-ANP in atrial extracts showed both high and low molecular weights. Serial dilutions of atrial extracts from chickens, turtles, frogs, and fish yielded competition curves which were parallel to the standard curve of AP III with antiserum No. 4. In comparison with two serial dilution curves of atrial extracts made using two antisera, No. 4 and No. 9, we suggest that the amino acid at position 12 of ir-ANP in the atrial extracts of chicken, turtle, frog, and fish is not isoleucine but is some other amino acid.

摘要

我们使用两种抗心房肽III（AP III）的抗血清，它们与心房利钠肽（ANP）类似物的交叉反应特性不同，测定了几种物种心脏提取物中的免疫反应性ANP（ir-ANP）。心房提取物中的ir-ANP显示出高分子量和低分子量。鸡、龟、蛙和鱼的心房提取物的系列稀释产生的竞争曲线与抗血清4的AP III标准曲线平行。与使用抗血清4和抗血清9制备的心房提取物的两条系列稀释曲线相比，我们认为鸡、龟、蛙和鱼的心房提取物中ir-ANP第12位的氨基酸不是异亮氨酸，而是其他某种氨基酸。