犬和兔血浆中心房利钠多肽的异源放射免疫分析

Heterologous radioimmunoassay of atrial natriuretic polypeptide in dog and rabbit plasma.

作者信息

Wilson N, Ledsome J R, Keeler R, Rankin A J, Wade J P, Courneya C A

出版信息

J Immunoassay. 1986;7(1-2):73-96. doi: 10.1080/01971528608063047.

DOI:10.1080/01971528608063047

Abstract

Atrial natriuretic peptide (ANP) was measured in plasma of dogs and rabbits by radioimmunoassay (RIA) using a commercially available anti alpha-ANP serum and compared to our measurements of ANP in rats and humans. Plasma concentration of ANP in dog coronary sinus (234.9 +/- 41.0 pg/ml) was significantly greater than in systemic arterial blood (81.2 +/- 8.4 pg/ml). Gel filtration of dog coronary sinus plasma resulted in an ANP peak with the elution volume (Ve) of synthetic atriopeptin III (AIII) and a minor peak eluting with the void volume (Vo). Rabbit systemic arterial plasma ANP was 53.3 +/- 4.3 pg/ml and yielded one peak, with a Ve of AIII. Ion exchange chromatography of dog and rabbit atrial extracts (AE) resulted in a major ANP region which resembled AIII. Gel filtration of AE showed larger molecular species as well as AIII. Dilutions of dog and rabbit plasma and AE were parallel with the AIII standard in radioimmunoassay.

摘要

使用市售的抗α-心钠素血清，通过放射免疫测定法（RIA）测定了犬和兔血浆中的心钠素（ANP），并与我们对大鼠和人类ANP的测量结果进行了比较。犬冠状窦血浆中ANP的浓度（234.9±41.0 pg/ml）显著高于体动脉血中的浓度（81.2±8.4 pg/ml）。对犬冠状窦血浆进行凝胶过滤，得到一个ANP峰，其洗脱体积（Ve）与合成心钠素III（AIII）相同，还有一个较小的峰在空体积（Vo）处洗脱。兔体动脉血浆ANP为53.3±4.3 pg/ml，产生一个峰，其Ve与AIII相同。对犬和兔心房提取物（AE）进行离子交换色谱分析，得到一个主要的ANP区域，与AIII相似。对AE进行凝胶过滤显示出较大分子的物质以及AIII。在放射免疫测定中，犬和兔血浆及AE的稀释液与AIII标准品呈平行关系。