Prasad D K V, Shaheen Uzma, Satyanarayana U, Surya Prabha T, Jyothy A, Munshi Anjana
Department of Molecular Biology, Institute of Genetics and Hospital for Genetic Diseases, Hyderabad, India.
Neurochem Res. 2014 Dec;39(12):2370-6. doi: 10.1007/s11064-014-1439-3. Epub 2014 Sep 26.
Certain minerals and trace elements are essential for the development of healthy nervous system. Altered serum levels of these elements may lead to the development of various diseases including epilepsy. The present study was designed to evaluate the association of serum calcium, magnesium, zinc and copper in the development of genetic generalized epilepsy [GGE; erstwhile known as idiopathic generalized epilepsy (IGE)] as well as idiopathic intractable epilepsy (IIE), in which seizures persist despite treatment with at least two or three antiepileptic drugs tolerated at reasonable dosage. 200 GGE patients and equal number of healthy controls were recruited for study with their written informed consent. The patients were further divided into responders and non-responders based on their response to antiepileptic drugs. Copper and zinc levels were assayed by atomic absorption spectrophotometer whereas calcium and magnesium were analyzed by Human Star 600 fully automated biochemistry analyzer. The patients with GGE had significant low levels of calcium, magnesium and zinc (1.85 ± 0.33, 0.69 ± 0.13 mmol/L and 11.33 ± 3.32 µmol/L respectively) and the corresponding values for controls were 2.27 ± 0.22, 0.89 ± 0.15, 12.71 ± 3.24 (p < 0.05). Significant high levels of copper were found in patients as compared to controls (26.69 ± 8.79 µmol/L; 16.64 ± 3.64) (p < 0.05). Significantly decreased levels of zinc were noted in non-responders (10.38 ± 2.99) compared to responders (12.62 ± 3.30) (p < 0.05). No significant difference was observed in serum calcium, magnesium and copper levels between responders and non-responders. In conclusion, low levels of calcium, magnesium, zinc and high levels of copper were found to be associated with GGE. Further, the patients with IIE were also found to have low levels of zinc.
某些矿物质和微量元素对健康神经系统的发育至关重要。这些元素血清水平的改变可能导致包括癫痫在内的各种疾病的发生。本研究旨在评估血清钙、镁、锌和铜与遗传性全身性癫痫[GGE；曾被称为特发性全身性癫痫（IGE）]以及特发性难治性癫痫（IIE）发生之间的关联，其中IIE是指尽管使用至少两三种合理剂量可耐受的抗癫痫药物治疗，但癫痫发作仍持续。本研究招募了200名GGE患者和数量相等的健康对照，并获得了他们的书面知情同意。根据患者对抗癫痫药物的反应，进一步将其分为反应者和无反应者。铜和锌水平通过原子吸收分光光度计测定，而钙和镁则通过Human Star 600全自动生化分析仪进行分析。GGE患者的钙、镁和锌水平显著降低（分别为1.85±0.33、0.69±0.13mmol/L和11.33±3.32µmol/L），对照组的相应值分别为2.27±0.22、0.89±0.15、12.71±3.24（p<0.05）。与对照组相比，患者的铜水平显著升高（26.69±8.79µmol/L；16.64±3.64）（p<0.05）。与反应者（12.62±3.30）相比，无反应者的锌水平显著降低（10.38±2.99）（p<0.05）。反应者和无反应者之间的血清钙、镁和铜水平未观察到显著差异。总之，发现钙、镁、锌水平低和铜水平高与GGE有关。此外，还发现IIE患者的锌水平也较低。
