Sepúlveda Lilian, Bussalleu Eva, Yeste Marc, Bonet Sergi
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071 Girona, Catalonia, Spain.
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071 Girona, Catalonia, Spain.
Anim Reprod Sci. 2014 Nov 30;150(3-4):96-106. doi: 10.1016/j.anireprosci.2014.09.001. Epub 2014 Sep 17.
Bacteriospermia in boar ejaculates is a frequent finding that compromises the sperm quality and, consequently, causes economic losses in swine industry. The present study sought to evaluate the effect of different concentrations of Pseudomonas aeruginosa on boar sperm quality over a storing period of 11 days at 15-17 ° C. Ten commercial seminal doses coming from post-pubertal and healthy boars were artificially inoculated with different infective concentrations of P. aeruginosa, ranging from 2 × 10(8) to 2 × 10(4)cfu/mL. Negative controls were non-inoculated doses. Sperm quality, assessed as sperm motility (CASA), sperm viability, acrosome integrity and pH, as well as the bacterial growth, were checked after 0, 1, 2, 4, 7, 9 and 11 days of storage at 15-17 ° C. Results obtained showed significant decreases in the percentages of total and progressive sperm motility, sperm viability and acrosome integrity in the greatest infective concentrations (2 × 10(7) and 2 × 10(8)cfu/mL), when compared to the negative control. In contrast, there was no effect on seminal pH throughout the experiment. Results indicate the presence of P. aeruginosa in boar semen, apart from being a potential source for the spread of infectious diseases and harmful impact on sows, negatively affects the longevity and fertilizing ability of boar sperm when present in high concentrations. Thus, P. aeruginosa causes deleterious effects on boar sperm quality during liquid storage at 15-17 ° C, thus strict hygienic measures must be implemented in boar studs to minimize bacterial concentration of semen doses.
公猪精液中的细菌污染是一个常见现象，会损害精子质量，进而给养猪业带来经济损失。本研究旨在评估不同浓度的铜绿假单胞菌在15-17°C的储存期为11天的情况下对公猪精子质量的影响。从青春期后的健康公猪采集的10剂商业精液，人工接种不同感染浓度的铜绿假单胞菌，范围从2×10(8)到2×10(4)cfu/mL。阴性对照为未接种的精液。在15-17°C储存0、1、2、4、7、9和11天后，检查精子质量（通过计算机辅助精子分析评估精子活力）、精子活力、顶体完整性和pH值，以及细菌生长情况。结果显示，与阴性对照相比，在最高感染浓度（2×10(7)和2×10(8)cfu/mL）下，总精子活力和渐进性精子活力、精子活力和顶体完整性的百分比显著下降。相比之下，在整个实验过程中，对精液pH值没有影响。结果表明，公猪精液中存在铜绿假单胞菌，除了是传染病传播的潜在来源以及对母猪有有害影响外，当高浓度存在时，还会对公猪精子的寿命和受精能力产生负面影响。因此，铜绿假单胞菌在15-17°C液体储存期间会对公猪精子质量产生有害影响，因此必须在公猪站实施严格的卫生措施，以尽量减少精液剂量中的细菌浓度。
