Luther Anne-Marie, Beckermann Christina, Nguyen Thu Quynh, Verspohl Jutta, Waberski Dagmar
Unit for Reproductive Medicine, Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Foundation, Bünteweg 15, D-30559 Hannover, Germany.
Institute for Microbiology, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Microorganisms. 2023 Mar 19;11(3):788. doi: 10.3390/microorganisms11030788.
The aim of this study was first to examine the prevalence of bacteria-associated loss of sperm quality in samples from insemination centers during a seven-year semen monitoring program and, second, to investigate the growth dynamic of four different multidrug-resistant bacterial species and their impact on sperm quality during semen storage. A reduced sperm quality associated with bacterial contamination was found in 0.5% of 3219 of the samples from insemination centers. In samples spiked with and bacterial growth by six log levels was seen during storage at 17 °C, causing loss of sperm motility, membrane integrity, membrane fluidity, and mitochondrial membrane potential at >10 CFU/mL ( < 0.05). Storage at 5 °C in the Androstar Premium extender efficiently inhibited their growth. and showed limited growth up to two log levels at 17 °C and did not impair sperm quality. In conclusion, spermatozoa tolerate moderate loads of multidrug-resistant bacteria, and hypothermic, antibiotic-free semen storage effectively limits bacterial growth. The constant use of antibiotics in semen extenders should be reconsidered.
本研究的目的，一是在一项为期七年的精液监测计划中，检测授精中心样本中与细菌相关的精子质量损失的发生率；二是研究四种不同的多重耐药细菌的生长动态及其在精液储存过程中对精子质量的影响。在授精中心的3219份样本中，有0.5%的样本发现精子质量下降与细菌污染有关。在添加了[具体细菌名称1]和[具体细菌名称2]的样本中，在17°C储存期间细菌生长达到六个对数水平，当细菌浓度>10 CFU/mL时导致精子活力、膜完整性、膜流动性和线粒体膜电位丧失（P<0.05）。在Androstar Premium稀释液中于5°C储存可有效抑制它们的生长。[具体细菌名称3]和[具体细菌名称4]在17°C时生长受限，最多达到两个对数水平，且不损害精子质量。总之，精子能够耐受中等负荷的多重耐药细菌，低温、无抗生素的精液储存可有效限制细菌生长。精液稀释液中抗生素的持续使用应重新考虑。
