Ren Nan, Zhang Shuyin, Li Yongfeng, Shen Shuning, Niu Qiang, Zhao Yimin, Kong Liang
State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China.
State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China.
Br J Oral Maxillofac Surg. 2014 Dec;52(10):907-12. doi: 10.1016/j.bjoms.2014.08.022. Epub 2014 Sep 23.
We investigated the response of rat bone mesenchymal stem cells (BMSC) placed on the titanium-6aluminium-7niobiuim (Ti-6Al-7Nb) alloy modified by hydrofluoric acid etch combined with subsequent anodic oxidation. Pure titanium (Ti) discs and Ti-6Al-7Nb discs were treated by hydrofluoric acid etch and anodic oxidation, and polished pure Ti discs and Ti-6Al-7Nb discs without surface modification served as controls (n=35 in each group). Scanning electron microscopy, atomic force microscopy, and radiographic photoelectron spectroscopy assays were used to detect the properties of the samples' surface. The morphology, adhesion, proliferation, and alkaline phosphatase activity of BMSC were examined using various techniques of microscopic and biological characterisation. The results showed that both Ti-6Al-7Nb samples and the pure Ti samples showed hierarchical micro/nanotopographies, and fluorine emerged on the surfaces of the samples after modification. The hierarchical micro/nanotopographies significantly increased the spreading, adhesion, and proliferation of BMSC and activity of alkaline phosphatase. In addition, modified samples of Ti-6Al-7Nb showed significantly higher alkaline phosphatase activity than modified pure Ti samples (p<0.05). The experiment successfully confirmed that Ti-6Al-7Nb alloy with hierarchical micro/nanotopographies treated by hydrofluoric acid etch and anodic oxidation possessed good biocompatibility, and may be a promising candidate for dental implants.
我们研究了置于经氢氟酸蚀刻并随后进行阳极氧化处理的钛-6铝-7铌（Ti-6Al-7Nb）合金上的大鼠骨髓间充质干细胞（BMSC）的反应。对纯钛（Ti）盘和Ti-6Al-7Nb盘进行氢氟酸蚀刻和阳极氧化处理，未进行表面改性的抛光纯Ti盘和Ti-6Al-7Nb盘作为对照（每组n = 35）。使用扫描电子显微镜、原子力显微镜和射线光电子能谱分析来检测样品表面的性质。使用各种微观和生物学表征技术检查BMSC的形态、粘附、增殖和碱性磷酸酶活性。结果表明，Ti-6Al-7Nb样品和纯Ti样品均呈现分级微/纳米拓扑结构，改性后样品表面出现氟元素。分级微/纳米拓扑结构显著增加了BMSC的铺展、粘附和增殖以及碱性磷酸酶活性。此外，Ti-6Al-7Nb改性样品的碱性磷酸酶活性显著高于纯Ti改性样品（p<0.05）。该实验成功证实，经氢氟酸蚀刻和阳极氧化处理的具有分级微/纳米拓扑结构的Ti-6Al-7Nb合金具有良好的生物相容性，可能是牙科植入物的一个有前景的候选材料。
