Khaw Juan Shong, Bowen Christopher R, Cartmell Sarah H
Department of Materials, The University of Manchester, Manchester M13 9PL, UK.
Department of Mechanical Engineering, University of Bath, Bath BA2 7AY, UK.
Nanomaterials (Basel). 2020 Oct 25;10(11):2117. doi: 10.3390/nano10112117.
The pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, including nanotube diameter. While research has been carried out in this area, there has yet to be a single systemic study to date that succinctly compares the response of both human stem cells and osteoblasts to a range of TiO nanotube pore diameters using controlled experiments in a single laboratory. In this paper, we investigate the influence of surface nanotopography on cellular behaviour and osseointegrative properties through a systemic study involving human mesenchymal stem cells (hMSCs) and human osteoblasts (HOBs) on TiO nanotubes of 20 nm, 50 nm and 100 nm pore diameters using in-vitro assessments. This detailed study demonstrates the interrelationship between cellular behaviour and nanotopography, revealing that a 20 nm nanotube pore diameter is preferred by hMSCs for the induction of osteogenic differentiation, while 50 nm nanotubular structures are favourable by HOBs for osteoblastic maturation.
结构均匀的纳米管的孔径可以显著改变细胞的行为。最近的研究表明，整合素的激活不仅受细胞-材料界面之间的表面化学影响，还受表面纳米拓扑结构的特征影响，包括纳米管直径。虽然该领域已经开展了相关研究，但迄今为止，尚未有一项单一的系统性研究能在单个实验室中通过对照实验简洁地比较人类干细胞和成骨细胞对一系列二氧化钛纳米管孔径的反应。在本文中，我们通过一项系统性研究，使用体外评估方法，研究了表面纳米拓扑结构对人骨髓间充质干细胞（hMSCs）和人成骨细胞（HOBs）在孔径为20纳米、50纳米和100纳米的二氧化钛纳米管上的细胞行为和骨整合特性的影响。这项详细研究证明了细胞行为与纳米拓扑结构之间的相互关系，揭示了hMSCs诱导成骨分化时更倾向于20纳米的纳米管孔径，而HOBs促进成骨细胞成熟时更青睐50纳米的纳米管结构。
