Rosseti Isabela Bueno, Taube Junior Paulo, de Campos Claudia Barbosa Ladeira, da Rocha Joao Batista Teixeira, Costa Maricilia Silva
Instituto de Pesquisa & Desenvolvimento, UNIVAP, Av. Shishima Hifumi 2911, CEP: 12244-000, São José dos Campos, Brazil.
Curr Drug Discov Technol. 2014;11(3):234-8. doi: 10.2174/1570163811666140924121758.
Candida species are the fourth most common cause of nosocomial bloodstream infections. An increase in the frequency of infections which have become refractory to standard antifungal therapyhave been observed. Recently, the effect of different organochalcogenide compounds reducing both growth and germ tube formation by Candida albicans was demonstrated. This work studied the effect of the organochalcogenide compound (pCl-PhSe)2 on both growth and biofilm formation by Candida albicans. A decrease in C. albicans growth in the presence of crescent concentrations of (pCl- PhSe)2 was observed, in a cell density dependent manner. The inhibition of Candida growth by 10µM (pCl-PhSe)2 was ~60, 57, 47 and 24%, in cell densities of 10(3), 10(4), 10(5) and 10(6) cells/ml, respectively. The compound (pCl-PhSe)2 was able to inhibit biofilm formation by Candida albicans, when biofilm was performed using a cell density of 10(6) cells/ml. In addition, an increase in both ROS production (96%) and cell membrane permeability (1.107-fold) by 10 µM (pCl-PhSe)2 was observed in C. albicans.These results demonstrate that the organochalcogenide compound (pCl-PhSe)2 presents a great potential to inhibit both growth and biofilm formation by C. albicans.
念珠菌属是医院血流感染的第四大常见病因。已观察到对标准抗真菌治疗难治的感染频率有所增加。最近，已证明不同的有机硫属化合物对白色念珠菌的生长和芽管形成均有抑制作用。这项工作研究了有机硫属化合物（pCl-PhSe）2对白色念珠菌生长和生物膜形成的影响。观察到在存在递增浓度的（pCl-PhSe）2时，白色念珠菌的生长呈细胞密度依赖性降低。在细胞密度分别为10³、10⁴、10⁵和10⁶个细胞/毫升时，10μM（pCl-PhSe）2对念珠菌生长的抑制率分别约为60%、57%、47%和24%。当使用10⁶个细胞/毫升的细胞密度形成生物膜时，化合物（pCl-PhSe）2能够抑制白色念珠菌生物膜的形成。此外，在白色念珠菌中观察到10μM（pCl-PhSe）2使活性氧生成增加（96%）和细胞膜通透性增加（1.107倍）。这些结果表明，有机硫属化合物（pCl-PhSe）2在抑制白色念珠菌生长和生物膜形成方面具有巨大潜力。
