Instituto de Pesquisa e Desenvolvimento - IP&D, Universidade do Vale do Paraíba - UNIVAP, São José dos Campos, SP, Brazil.
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, RS, Brazil.
Biofouling. 2021 Feb;37(2):235-245. doi: 10.1080/08927014.2021.1897110. Epub 2021 Mar 15.
Adhesion capacity on biological surfaces and biofilm formation is considered an important step in the infection process by The ability of (PhSe) and (Cl-PhSe), two synthetic organic selenium (organochalcogen) compounds, to act on virulence factors related to adhesion to human endocervical (HeLa) cell surfaces and their anti-biofilm activities was analyzed. Both organochalcogen compounds inhibited adhesion to HeLa cells, dependent on compound concentrations. (PhSe) (at 20 µM; = 0.0012) was significantly more effective than (Cl-PhSe) (at 20 µM; = 0.0183) compared with the control. (PhSe) inhibited biofilm formation and decreased biofilm viability in both early and mature biofilms more efficiently than (Cl-PhSe). Overall, the organochalcogen compounds, especially (PhSe), were demonstrated to be effective antifungal drugs against virulence factors related to epithelial cell surface adhesion and the formation and viability of biofilms.
生物表面的黏附能力和生物膜形成被认为是感染过程中的一个重要步骤。本研究分析了两种合成有机硒（有机硫属元素）化合物（PhSe）和（Cl-PhSe）对与黏附到人宫颈内（HeLa）细胞表面相关的毒力因子的作用及其抗生物膜活性。两种有机硫属元素化合物均依赖于化合物浓度抑制对 HeLa 细胞的黏附。与对照组相比，（PhSe）（在 20 μM 时； = 0.0012）比（Cl-PhSe）（在 20 μM 时； = 0.0183）更能显著抑制黏附。（PhSe）比（Cl-PhSe）更有效地抑制早期和成熟生物膜中的生物膜形成并降低生物膜活力。总体而言，有机硫属元素化合物，尤其是（PhSe），被证明是针对与上皮细胞表面黏附以及生物膜形成和活力相关的毒力因子的有效抗真菌药物。
