Inhibition of Development and Metabolism of Dual-Species Biofilms of and () by Organoselenium Compounds.

Although is the most frequently identified species in clinical settings, a significant number of infections related to the non- (NAC) species, , has been reported. Both species are able to produce biofilms and have been an important resistance-related factor to antimicrobial resistance. In addition, the microbial relationship is common in the human body, contributing to the formation of polymicrobial biofilms. Considering the great number of reports showing the increase in cases of resistance to the available antifungal drugs, the development of new and effective antifungal agents is critical. The inhibitory effect of Organoselenium Compounds (OCs) on the development of and was recently demonstrated, supporting the potential of these compounds as efficient antifungal drugs. In addition, OCs were able to reduce the viability and the development of biofilms, a very important step in colonization and infection caused by fungi. Thus, the objective of this study was to investigate the effect of the Organoselenium Compounds (-MeOPhSe), (PhSe) and (-Cl-PhSe) on the development of dual-species biofilms of and produced using either RPMI-1640 or Sabouraud Dextrose Broth (SDB) media. The development of dual-species biofilms was evaluated by the determination of both metabolic activity, using a metabolic assay based on the reduction of XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt) assay and identification of either and on CHROMagar Candida medium. Biofilm formation using RPMI-1640 was inhibited in 90, 55, and 20% by 30 µM (-MeOPhSe), (PhSe) and (-Cl-PhSe), respectively. However, biofilms produced using SDB presented an inhibition of 62, 30 and 15% in the presence of 30 µM (-MeOPhSe), (PhSe) and (-Cl-PhSe), respectively. The metabolic activity of 24 h biofilms was inhibited by 35, 30 and 20% by 30 µM (-MeOPhSe), (PhSe) and (-Cl-PhSe), respectively, with RPMI-1640; however, 24 h biofilms formed using SDB were not modified by the OCs. In addition, a great reduction in the number of CFUs of (93%) in biofilms produced using RPMI-1640 in the presence of 30 µM (-MeOPhSe) was observed. However, biofilms formed using SDB and treated with 30 µM (-MeOPhSe) presented a reduction of 97 and 69% in the number of CFUs of and , respectively. These results demonstrated that Organoselenium Compounds, mainly (-MeOPhSe) are able to decrease the metabolic activity of dual-species biofilms by reducing both and cell number during biofilm formation using either RPMI-1640 or SDB. Taken together, these results demonstrated the potential of the OCs to inhibit the development of dual-species biofilms of and .