Quinn Kyle P, Golberg Alexander, Broelsch G Felix, Khan Saiqa, Villiger Martin, Bouma Brett, Austen William G, Sheridan Robert L, Mihm Martin C, Yarmush Martin L, Georgakoudi Irene
Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
Exp Dermatol. 2015 Jan;24(1):78-80. doi: 10.1111/exd.12553. Epub 2014 Nov 11.
Standard approaches to evaluate scar formation within histological sections rely on qualitative evaluations and scoring, which limits our understanding of the remodelling process. We have recently developed an image analysis technique for the rapid quantification of fibre alignment at each pixel location. The goal of this study was to evaluate its application for quantitatively mapping scar formation in histological sections of cutaneous burns. To this end, we utilized directional statistics to define maps of fibre density and directional variance from Masson's trichrome-stained sections for quantifying changes in collagen organization during scar remodelling. Significant increases in collagen fibre density are detectable soon after burn injury in a rat model. Decreased fibre directional variance in the scar was also detectable between 3 weeks and 6 months after injury, indicating increasing fibre alignment. This automated analysis of fibre organization can provide objective surrogate endpoints for evaluating cutaneous wound repair and regeneration.
评估组织学切片内瘢痕形成的标准方法依赖于定性评估和评分，这限制了我们对重塑过程的理解。我们最近开发了一种图像分析技术，用于快速量化每个像素位置的纤维排列。本研究的目的是评估其在定量绘制皮肤烧伤组织学切片中瘢痕形成的应用。为此，我们利用方向统计从Masson三色染色切片定义纤维密度和方向方差图，以量化瘢痕重塑过程中胶原组织的变化。在大鼠模型中，烧伤损伤后不久即可检测到胶原纤维密度显著增加。在损伤后3周和6个月之间也可检测到瘢痕中纤维方向方差降低，表明纤维排列增加。这种对纤维组织的自动分析可为评估皮肤伤口修复和再生提供客观的替代终点。
