College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
Int J Biol Macromol. 2015 Jan;72:1182-90. doi: 10.1016/j.ijbiomac.2014.08.058. Epub 2014 Sep 23.
Optimization of extraction conditions, preliminary characterization and in vitro antioxidant activity of polysaccharides from black soybean (BSPS) were investigated. The results of Box-Behnken design showed that the optimal extraction conditions for BSPS were as follows: ratio of water to material of 20 ml/g, extraction time of 6.4h and extraction temperature of 92 °C, with a corresponding yield of 2.56%. The crude BSPS were further fractionated on DEAE-52 and Sepharose CL-4B chromatography to afford three purified fractions (BSPS-1, BSPS-2 and BSPS-3). Chemical analysis showed that the three purified fractions were mainly composed of carbohydrate and uronic acid. In addition, BSPS-1 was composed of arabinose, rhamnose, galactose, glucose and mannose in the molar ratio of 1.79:1.00:2.59:26.54:1.01. BSPS-2 was composed of arabinose, rhamnose, xylose, galactose and mannose in the molar ratio of 8.10:4.80:9.15:13.38:1.00. BSPS-3 was composed of arabinose, rhamnose, galactose and mannose in the molar ratio of 16.80:3.60:33.66:1.00. The results of Fourier transform-infrared spectroscopy further confirmed the characteristic polysaccharide structures of the three purified fractions. Moreover, antioxidant assays showed crude BSPS and its purified fractions had potential superoxide anion and DPPH radical scavenging activities, and their antioxidant activity decreased in the order of crude BSPS > BSPS-3 > BSPS-2 > BSPS-1.
优化了黑豆（BSPS）多糖的提取条件、初步表征和体外抗氧化活性。Box-Behnken 设计结果表明，BSPS 的最佳提取条件为：料液比 20ml/g、提取时间 6.4h、提取温度 92°C，相应得率为 2.56%。粗 BSPS 进一步经 DEAE-52 和 Sepharose CL-4B 层析分离，得到 3 个纯化级分（BSPS-1、BSPS-2 和 BSPS-3）。化学分析表明，这 3 个纯化级分主要由碳水化合物和糖醛酸组成。此外，BSPS-1 由阿拉伯糖、鼠李糖、半乳糖、葡萄糖和甘露糖以摩尔比 1.79:1.00:2.59:26.54:1.01 组成。BSPS-2 由阿拉伯糖、鼠李糖、木糖、半乳糖和甘露糖以摩尔比 8.10:4.80:9.15:13.38:1.00 组成。BSPS-3 由阿拉伯糖、鼠李糖、半乳糖和甘露糖以摩尔比 16.80:3.60:33.66:1.00 组成。傅里叶变换红外光谱分析进一步证实了这 3 个纯化级分的特征多糖结构。此外，抗氧化测定表明，粗 BSPS 及其纯化级分具有潜在的超氧阴离子和 DPPH 自由基清除活性，其抗氧化活性顺序为粗 BSPS>BSPS-3>BSPS-2>BSPS-1。
