Nagavalli M, Ponamgi S P D, Girijashankar V, Venkateswar Rao L
Department of Microbiology, Osmania University, Hyderabad, India.
Lett Appl Microbiol. 2015 Jan;60(1):44-51. doi: 10.1111/lam.12332. Epub 2014 Nov 16.
Production of Rifamycin SV from cheaper agro-industrial by-products using mutant strain of Amycolatopsis mediterranei OVA5-E7 in solid state fermentation (SSF) was optimized. Among the agro-based substrates used, ragi bran was found suitable for maximizing the yield of Rifamycin SV (1310 mg 100 g(-1) ds). The yield can be further enhanced to 19·7 g Kg(-1) of dry substrate by supplementing the substrate with deoiled cotton cake (10% w/w) using optimized fermentation parameters such as maintaining 80% moisture, pH 7·0, 30°C incubation temperature, inoculum 25% v/w and carrying the solid state fermenting for 9 days. Manipulating these seven specifications, the end product yield achieved in our experimentation was 20 g of Rifamycin SV Kg(-1) ds. Eventually, an overall 5-fold improvement in Rifamycin SV production was achieved.
Antibiotics such as rifamycin are broad-spectrum antimicrobial drugs used in large-scale worldwide as human medicine towards controlling diseases. Amycolatopsis mediterranei strain which produces this antibiotic was earlier used in submerged fermentation yielded lower amounts of rifamycin. By employing cheaper agro-industrial by-products, we produced upto 20 g rifamycin SV per Kg dry substrate used under optimized solid state fermentation conditions. Keeping in view, the role of rifamycin in meeting the medical demands of world's increasing population; we successfully used an improved strain on cheaper substrates with optimized fermentation parameters and achieved a 5-fold improvement in rifamycin SV production.
利用地中海拟无枝酸菌OVA5 - E7突变株在固态发酵（SSF）中从更廉价的农业工业副产品生产利福霉素SV的过程得到了优化。在所使用的基于农业的底物中，发现ragi麸皮适合使利福霉素SV的产量最大化（1310毫克/100克干物质）。通过使用优化的发酵参数，如保持80%的湿度、pH值7.0、30°C的培养温度、25%（体积/重量）的接种量以及进行9天的固态发酵，向底物中添加脱油棉籽饼（10%重量/重量），产量可进一步提高到19.7克/千克干底物。通过控制这七个参数，我们实验中获得的最终产品产量为20克利福霉素SV/千克干物质。最终，利福霉素SV的产量总体提高了5倍。
利福霉素等抗生素是在全球范围内作为人类药物用于控制疾病的广谱抗菌药物。 earlier用于深层发酵的地中海拟无枝酸菌菌株产生的利福霉素量较低。通过使用更廉价的农业工业副产品，我们在优化的固态发酵条件下，每千克干底物最多可生产20克利福霉素SV。考虑到利福霉素在满足世界不断增长的人口的医疗需求方面的作用；我们成功地在更廉价的底物上使用改良菌株并采用优化的发酵参数，使利福霉素SV的产量提高了5倍。 （注：原文中“earlier used in submerged fermentation yielded lower amounts of rifamycin”表述似乎不太完整通顺，可能存在一定语病，但按要求未做修改直接翻译。）
