Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St, POB: 11566, Cairo, Abbassia, Egypt.
BMC Microbiol. 2021 Jan 23;21(1):34. doi: 10.1186/s12866-021-02093-6.
Paromomycin is a 2-deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation.
Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. Optimization of environmental conditions using D-optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C.
Compared to submerged liquid fermentation, solid state fermentation resulted in comparable paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, solid state fermentation is a promising alternative to submerged liquid fermentation for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.
巴龙霉素是一种 2-脱氧链霉胺氨基环醇氨基糖苷抗生素，对革兰氏阴性、革兰氏阳性细菌和许多原生动物具有广谱活性。本研究介绍了一种使用链霉菌雷莫菌亚种。通过固态发酵生产巴龙霉素的策略。与深层液体发酵相比，固态发酵在许多产品的开发中引起了极大的关注，因为它具有许多优势。在选择最佳固体基质后，进行了巴龙霉素生产的时间过程研究，然后使用响应面法对环境条件进行了优化。还将使用该技术获得的巴龙霉素产量与使用深层液体发酵获得的产量进行了比较。
在筛选了 6 种不同的基质后，用具有成本效益的农业工业副产品玉米麸皮，用氨基糖苷生产培养基浸渍，获得了最大的巴龙霉素浓度（0.51 mg/g 初始干固体）。使用 D-最优设计对环境条件进行优化，使巴龙霉素浓度提高了 4.3 倍，达到 2.21 mg/g 初始干固体，pH 值为 8.5，接种量为 5%（v/w），温度为 30°C。
与深层液体发酵相比，固态发酵可获得可比的巴龙霉素浓度，降低原材料成本，减少能源消耗和废水排放，这对工业发酵具有重大意义。因此，固态发酵是生产巴龙霉素的一种很有前途的替代深层液体发酵的方法。据我们所知，这是第一篇关于通过固态发酵过程优化巴龙霉素生产的报告。
