Zauner Dorothea, Quehenberger Franz, Hermann Josef, Dejaco Christian, Stradner Martin H, Stojakovic Tatjana, Angerer Hannes, Rinner Beate, Graninger Winfried B
Department of Rheumatology and Immunology, Medical University , Graz .
Int J Hyperthermia. 2014 Sep;30(6):393-401. doi: 10.3109/02656736.2014.956810.
Exposure to increased environmental temperatures is commonly used as a non-pharmacological treatment modality in ankylosing spondylitis (AS). We aimed to investigate systemic immunological effects of moderate whole body hyperthermia in patients with AS compared to healthy control subjects.
Ten healthy control subjects and six AS patients underwent whole body hyperthermia treatment with 38.7-39 °C body core temperature over 60 min. Numbers of polymorphonuclear leucocytes and lymphocyte subsets, plasma concentrations of several acute phase reactants and cytokines, and gene expression levels of toll-like receptor 4 (TLR-4), interleukin 10 (IL-10) and heat shock protein beta 1 (HSPB1) were determined during and up to 24 h after treatment.
TLR-4, IL-10 and HSPB1 gene expression increased significantly up to 3 h post treatment, with an earlier, higher and more pronounced increase of IL-10 in patients with AS. An increase of natural killer cells and CD8+ T lymphocytes was noted during active heating, with a subsequent decrease up to 2 h after treatment. CD4+ T lymphocytes showed a short increase during active treatment in AS patients, while decreasing immediately after start of treatment in control subjects. Neutrophil granulocytes increased significantly up to 3 h after treatment, monocytes and B lymphocytes remained unchanged. Likewise, no significant changes were found concerning systemic cytokine concentrations and acute phase reactants.
Our data support the concept of systemic immunological effects of moderate whole body hyperthermia in patients with AS.
暴露于升高的环境温度下通常被用作强直性脊柱炎（AS）的一种非药物治疗方式。我们旨在研究与健康对照受试者相比，中度全身热疗对AS患者的全身免疫效应。
10名健康对照受试者和6名AS患者接受了全身热疗，体温维持在38.7 - 39°C，持续60分钟。在治疗期间及治疗后24小时内，测定多形核白细胞和淋巴细胞亚群的数量、几种急性期反应物和细胞因子的血浆浓度，以及Toll样受体4（TLR - 4）、白细胞介素10（IL - 10）和热休克蛋白β1（HSPB1）的基因表达水平。
治疗后3小时内，TLR - 4、IL - 10和HSPB1基因表达显著增加，AS患者中IL - 10的增加更早、更高且更明显。在主动加热期间，自然杀伤细胞和CD8 + T淋巴细胞数量增加，随后在治疗后2小时内减少。AS患者在主动治疗期间CD4 + T淋巴细胞短暂增加，而对照受试者在治疗开始后立即减少。中性粒细胞在治疗后3小时内显著增加，单核细胞和B淋巴细胞保持不变。同样，全身细胞因子浓度和急性期反应物未发现显著变化。
我们的数据支持中度全身热疗对AS患者具有全身免疫效应的概念。
