Riedel R, Marrassini C, Anesini C, Gorzalczany S
Cátedra de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
Phytother Res. 2015 Jan;29(1):59-66. doi: 10.1002/ptr.5226. Epub 2014 Sep 24.
Urera aurantiaca Wedd. (Urticaceae) is a medicinal plant commonly used in traditional medicine to relieve pain in inflammatory processes. In the present study, the in vivo anti-inflammatory and antinociceptive effects of U. aurantiaca methanolic extract and its possible mechanisms of action were investigated. The extract showed anti-inflammatory activity in the ear edema in mice test (34.3% inhibition), myeloperoxidase (MPO) activity was markedly reduced in animals administered with the extract: within 49.6% and 68.5%. In the histological analysis, intense dermal edema and intense cellular infiltration of inflammatory cells were markedly reduced in the ear tissue of the animals treated with the extract. In the carrageenan-induced hind paw edema in rats assay the extract provoked a significant inhibition of the inflammation (45.5%, 5 h after the treatment) and the MPO activity was markedly reduced (maximum inhibition 71.7%), The extract also exhibited significant and dose-dependent inhibitory effect on the increased vascular permeability induced by acetic acid. The extract presented antioxidant activity in both 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis 3-ethylbenzothiazoline 6-sulfonic acid tests and its total phenol content was 35.4 ± 0.06 mg GAE/g of extract. Also, the extract produced significant inhibition on nociception induced by acetic acid (ED50 : 8.7 mg/kg, i.p.) administered intraperitoneally and orally. Naloxone significantly prevented this activity.
橙黄艾麻（荨麻科）是一种药用植物，在传统医学中常用于缓解炎症过程中的疼痛。在本研究中，对橙黄艾麻甲醇提取物的体内抗炎和抗伤害感受作用及其可能的作用机制进行了研究。该提取物在小鼠耳部水肿试验中显示出抗炎活性（抑制率为34.3%），给予提取物的动物体内髓过氧化物酶（MPO）活性显著降低：降低幅度在49.6%至68.5%之间。在组织学分析中，提取物处理动物耳部组织的真皮水肿和炎症细胞的强烈浸润明显减少。在角叉菜胶诱导的大鼠后爪水肿试验中，提取物对炎症有显著抑制作用（治疗后5小时抑制率为45.5%），MPO活性显著降低（最大抑制率为71.7%），提取物对乙酸诱导的血管通透性增加也表现出显著的剂量依赖性抑制作用。该提取物在2,2-二苯基-1-苦基肼和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）试验中均呈现抗氧化活性，其总酚含量为35.4±0.06毫克没食子酸当量/克提取物。此外，该提取物对腹腔注射和口服乙酸诱导的伤害感受有显著抑制作用（半数有效剂量：8.7毫克/千克，腹腔注射）。纳洛酮可显著阻止这种活性。
