Biomedical Engineering and Physics, ‡ Laboratory of Experimental Clinical Chemistry, Academic Medical Center, University of Amsterdam , Amsterdam, The Netherlands.
Nano Lett. 2014 Nov 12;14(11):6195-201. doi: 10.1021/nl503371p. Epub 2014 Oct 2.
The refractive index (RI) dictates interaction between light and nanoparticles and therefore is important to health, environmental, and materials sciences. Using nanoparticle tracking analysis, we have determined the RI of heterogeneous particles <500 nm in suspension. We demonstrate feasibility of distinguishing silica and polystyrene beads based on their RI. The hitherto unknown RI of extracellular vesicles from human urine was determined at 1.37 (mean). This method enables differentiation of single nanoparticles based on their RI.
折射率（RI）决定了光与纳米粒子的相互作用，因此对健康、环境和材料科学都很重要。我们使用纳米颗粒跟踪分析技术，确定了悬浮液中 <500nm 的异质颗粒的 RI。我们证明了基于 RI 区分二氧化硅和聚苯乙烯珠的可行性。人类尿液中细胞外囊泡的 RI 此前未知，为 1.37（平均值）。该方法能够基于 RI 对单个纳米颗粒进行区分。
