Saul Nadine, Chakrabarti Shumon, Stürzenbaum Stephen R, Menzel Ralph, Steinberg Christian E W
Department of Biology, Freshwater and Stress Ecology, Humboldt-Universität zu Berlin, Späthstr. 80/81, 12437 Berlin, Germany.
Department of Biology, Freshwater and Stress Ecology, Humboldt-Universität zu Berlin, Späthstr. 80/81, 12437 Berlin, Germany.
Chem Biol Interact. 2014 Nov 5;223:51-7. doi: 10.1016/j.cbi.2014.09.007. Epub 2014 Sep 23.
Cyanobacterial blooms in aquatic environments are frequently characterized by elevated levels of microcystins, a potent hepatotoxin. Here we exposed the nematode Caenorhabditis elegans with environmentally realistic concentrations of MC-LR to explore its non-hepatic toxicity. Lifespan, reproduction and growth assays confirmed the toxic potential of 100μg/L MC-LR even in this liver-lacking invertebrate. Whole-genome microarray analysis revealed that a neuromodulating action was the dominant response in nematodes challenged with 100μg/L MC-LR. Indeed, most of the 201 differentially expressed genes were associated with neurobehavior, neurogenesis, and signaling associated pathways. In addition, a whole-genome miRNA-microarray highlighted that, in particular, members of the let-7 family were differentially regulated. These miRNAs are involved in the developmental timing of cell fates, including neurons, and are probably also part of the stress response system. To conclude, neurological modulation is the main transcriptional stress response in C. elegans exposed to MC-LR.
水生环境中的蓝藻水华通常以微囊藻毒素水平升高为特征，微囊藻毒素是一种强效肝毒素。在此，我们用环境实际浓度的MC-LR处理线虫秀丽隐杆线虫，以探究其非肝脏毒性。寿命、繁殖和生长试验证实，即使在这种无肝脏的无脊椎动物中，100μg/L的MC-LR也具有毒性潜力。全基因组微阵列分析表明，神经调节作用是受100μg/L MC-LR刺激的线虫中的主要反应。实际上，201个差异表达基因中的大多数与神经行为、神经发生及相关信号通路有关。此外，全基因组miRNA微阵列突出显示，特别是let-7家族成员受到差异调节。这些miRNA参与包括神经元在内的细胞命运的发育时间调控，可能也是应激反应系统的一部分。总之，神经调节是暴露于MC-LR的秀丽隐杆线虫中的主要转录应激反应。
