Leonard Davis School of Gerontology, University of Southern California, Los Angeles.
Department of Biomedical Sciences, Center for Bioinformatics and Genomics, Cedars-Sinai Medical Center, Los Angeles, California.
J Gerontol A Biol Sci Med Sci. 2019 Jul 12;74(8):1189-1197. doi: 10.1093/gerona/glz063.
Air pollution is a heterogeneous environmental toxicant that impacts humans throughout their life. We introduce Caenorhabditis elegans as a valuable air pollution model with its short lifespan, medium-throughput capabilities, and highly conserved biological pathways that impact healthspan. We exposed developmental and adult life stages of C. elegans to airborne nano-sized particulate matter (nPM) produced by traffic emissions and measured biological and molecular endpoints that changed in response. Acute nPM did not cause lethality in C. elegans, but short-term exposure during larval stage 1 caused delayed development. Gene expression responses to nPM exposure overlapped with responses of mouse and cell culture models of nPM exposure in previous studies. We showed further that the skn-1/Nrf2 antioxidant response has a role in the development and hormetic effects of nPM. This study introduces the worm as a new resource and complementary model for mouse and cultured cell systems to study air pollution toxicity across the lifespan.
空气污染是一种异质的环境毒物，会影响人类的一生。我们引入秀丽隐杆线虫作为一种有价值的空气污染模型，因为它具有短的生命周期、中等通量的能力，以及高度保守的影响健康寿命的生物途径。我们将发育和成年阶段的秀丽隐杆线虫暴露于交通排放产生的空气传播纳米级颗粒物（nPM）中，并测量了响应变化的生物学和分子终点。急性 nPM 不会导致秀丽隐杆线虫死亡，但在幼虫 1 期的短期暴露会导致发育迟缓。nPM 暴露的基因表达反应与先前研究中 nPM 暴露的小鼠和细胞培养模型的反应重叠。我们进一步表明，skn-1/Nrf2 抗氧化反应在 nPM 的发育和毒物兴奋效应中起作用。这项研究将秀丽隐杆线虫作为一种新的资源和补充模型引入到小鼠和培养细胞系统中，以研究整个生命周期中的空气污染毒性。
