Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstrasse 31, 93053, Regensburg, Germany.
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstrasse 31, 93053, Regensburg, Germany.
Anal Chim Acta. 2019 May 30;1057:18-35. doi: 10.1016/j.aca.2018.11.042. Epub 2018 Nov 25.
For the identification and quantification of analytes in complex samples, highly selective analytical strategies are required. The selectivity of single separation techniques such as gas chromatography (GC), liquid chromatography (LC), or capillary electrophoresis (CE) with common detection principles can be enhanced by hyphenating orthogonal separation techniques but also by using complementary detection systems. In this review, two-dimensional systems containing CE in at least one dimension are reviewed, namely LC-CE or 2D CE systems. Particular attention is paid to the aspect of selectivity enhancement due to the orthogonality of the different separation mechanisms. As an alternative concept, dual detection approaches are reviewed using the common detectors of CE such as UV/VIS, laser-induced fluorescence, capacitively coupled contactless conductivity (CD), electrochemical detection, and mass spectrometry. Special emphasis is given to dual detection systems implementing the highly flexible CD as one detection component. Selectivity enhancement can be achieved in case of complementarity of the different detection techniques.
为了鉴定和定量分析复杂样本中的分析物，需要高度选择性的分析策略。通过将正交分离技术与互补检测系统相结合，可以增强诸如气相色谱（GC）、液相色谱（LC）或毛细管电泳（CE）等常见检测原理的单一分离技术的选择性。在这篇综述中，我们回顾了至少在一维中包含 CE 的二维系统，即 LC-CE 或 2DCE 系统。特别关注由于不同分离机制的正交性而导致的选择性增强方面。作为替代概念，我们还回顾了使用 CE 的常见检测器（如 UV/VIS、激光诱导荧光、容性耦合非接触式电导（CD）、电化学检测和质谱）的双检测方法。特别强调了将高度灵活的 CD 作为一个检测组件的双检测系统。在不同检测技术互补的情况下，可以实现选择性增强。
