间充质干细胞脑内移植对老年大鼠软脑膜小动脉反应性的影响。

Effect of intracerebral transplantation of mesenchymal stem cells on reactivity of pial arterioles in old rats.

作者信息

Sokolova I B, Sergeev I V, Anisimov S V, Puzanov M V, Dvoretskii D P

机构信息

Laboratory of Physiology of Circulation, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia,

出版信息

Bull Exp Biol Med. 2014 Sep;157(5):663-5. doi: 10.1007/s10517-014-2639-1. Epub 2014 Sep 26.

DOI:10.1007/s10517-014-2639-1

PMID:25257436

Abstract

Using a TV device for studying microcirculation (×160), we analyzed the responses of arterioles in the pia mater of the sensorimotor cortex in young (2-3 months) and old (22-24 months) rats after local application of a vasoconstrictor (norepinephrine, 10(-6) M) or vasodilator (acetylcholine, 10(-6) M). The responses of the arterioles were evaluated by changes in their diameter and by the number of responding vessels in the field of view. The constrictor responses of the pial arteries to norepinephrine did not significantly differ in intact young and old rats. The number and degree of dilatory responses to acetylcholine in old rats were lower than in young animals by 14 and 30%, respectively. Intracerebral transplantation of mesenchymal stem cells to old rats had practically no effect on reactivity of pial arterioles to acetylcholine, while the number of constricted vessels in response to norepinephrine increased by ~20%.

摘要

使用电视设备研究微循环（×160），我们分析了年轻（2 - 3个月）和老年（22 - 24个月）大鼠在局部应用血管收缩剂（去甲肾上腺素，10^(-6) M）或血管扩张剂（乙酰胆碱，10^(-6) M）后，感觉运动皮层软脑膜中小动脉的反应。通过小动脉直径的变化和视野中反应性血管的数量来评估小动脉的反应。在完整的年轻和老年大鼠中，软脑膜动脉对去甲肾上腺素的收缩反应没有显著差异。老年大鼠对乙酰胆碱的扩张反应的数量和程度分别比年轻动物低14%和30%。向老年大鼠脑内移植间充质干细胞对软脑膜小动脉对乙酰胆碱的反应性几乎没有影响，而对去甲肾上腺素反应而收缩的血管数量增加了约20%。