Zhang Min, Zhou Jing, Wang Jingchun, Zhou Quan, Fang Jin, Zhou Chengqian, Chen WenLi
MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, China.
Biotechnol Lett. 2015 Feb;37(2):289-98. doi: 10.1007/s10529-014-1681-4. Epub 2014 Sep 26.
Immature dendritic cells (iDCs) are for cell transplantation; however, no method has yet been developed for in vivo monitoring the transplanted iDCs. We have explored the feasibility of using superparamagnetic iron oxide (SPIO) labeling and magnetic resonance imaging for in vivo tracking of transplanted iDCs and determined the effects of SPIO labeling on iDC vaccination. With up to 50 μg Fe/ml, SPIO effectively labeled the iDCs without affecting their growth. At or above 100 μg Fe/ml, SPIO caused considerable damage to iDCs. SPIO labeling resulted in autophagosome formation and decreased the uptake of oxidized low density lipoprotein (ox-LDL), an exogenous antigen, by iDCs. SPIO and ox-LDL both localized to the lysosomes, and this competition for lysosomes could be partially responsible for the decreased ox-LDL phagocytic capacity of iDCs due to SPIO labeling.
未成熟树突状细胞（iDCs）适用于细胞移植；然而，尚未开发出用于在体内监测移植的iDCs的方法。我们探讨了使用超顺磁性氧化铁（SPIO）标记和磁共振成像在体内追踪移植的iDCs的可行性，并确定了SPIO标记对iDC疫苗接种的影响。当铁浓度高达50μg/ml时，SPIO有效地标记了iDCs，且不影响其生长。当铁浓度达到或高于100μg/ml时，SPIO对iDCs造成了相当大的损害。SPIO标记导致自噬体形成，并降低了iDCs对氧化低密度脂蛋白（ox-LDL，一种外源性抗原）的摄取。SPIO和ox-LDL都定位于溶酶体，这种对溶酶体的竞争可能是SPIO标记导致iDCs对ox-LDL吞噬能力下降的部分原因。
