Zhang Wei, Zhang Shuihua, Xu Wan, Zhang Min, Zhou Quan, Chen Wenli
MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, China.
Medical Imaging Center, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Biotechnol Lett. 2017 Jul;39(7):1079-1089. doi: 10.1007/s10529-017-2332-3. Epub 2017 Apr 10.
To investigate the effects of ultrasmall superparamagnetic iron oxide (USPIO) labeling on the maturity or immune tolerance of immature dendritic cells (imDCs) as the success of immunotherapy with immature dendritic cells is highly dependent on immune tolerance.
The feasibility of tracking implanted USPIO-labeled imDCs in vivo by magnetic resonance imaging (MRI) was explored. The effects of USPIO labeling on the immune tolerance of imDCs was examined. USPIO when higher than 200 μg/ml caused considerable damage to imDCs, induced imDC maturation, and impacted the immune tolerance of imDCs. USPIO labeling caused a dose-dependent increase in autophagosome formation in imDCs, and autophagy inhibitors prevented the maturation of imDCs while stimulating their immune tolerance.
We speculate that high concentrations of USPIO can be used to induce imDC maturation, and that this process is likely mediated through an autophagy-related pathway.
研究超小超顺磁性氧化铁（USPIO）标记对未成熟树突状细胞（imDCs）成熟度或免疫耐受性的影响，因为未成熟树突状细胞免疫治疗的成功高度依赖于免疫耐受性。
探索了通过磁共振成像（MRI）在体内追踪植入的USPIO标记的imDCs的可行性。检测了USPIO标记对imDCs免疫耐受性的影响。当USPIO高于200μg/ml时，对imDCs造成相当大的损害，诱导imDCs成熟，并影响imDCs的免疫耐受性。USPIO标记导致imDCs中自噬体形成呈剂量依赖性增加，自噬抑制剂可阻止imDCs成熟，同时刺激其免疫耐受性。
我们推测高浓度的USPIO可用于诱导imDCs成熟，并且该过程可能通过自噬相关途径介导。
