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合成超顺磁性氧化铁对树突状细胞的影响。

Influence of synthetic superparamagnetic iron oxide on dendritic cells.

机构信息

Central Laboratory of Stomatology, Stomatological Hospital Affiliated Medical School, Nanjing University, Nanjing 210008, People’s Republic of China.

出版信息

Int J Nanomedicine. 2011;6:1779-86. doi: 10.2147/IJN.S23240. Epub 2011 Aug 25.

DOI:10.2147/IJN.S23240
PMID:21980240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3184937/
Abstract

BACKGROUND

This study investigated the influence of synthetic superparamagnetic iron oxide (SPIO) on dendritic cells and provides a possible method for labeling these cells.

METHODS

SPIO nanoparticles were prepared, and their morphology and magnetic properties were characterized. The particles were endocytosed by dendritic cells generated from mouse bone marrow. Labeling efficiency and cellular uptake were analyzed by Prussian blue staining and quantitative spectrophotometric assay. Meanwhile, the surface molecules, cellular apoptosis, and functional properties of the SPIO-labeled dendritic cells were explored by flow cytometry and the mixed lymphocyte reaction assay.

RESULTS

The synthetic nanoparticles possessed a spherical shape and good superparamagnetic behavior. The mean concentration of iron in immature and mature dendritic cells was 31.8 ± 0.7 μg and 35.6 ± 1.0 μg per 1 × 10(6) cells, respectively. After 12 hours of incubation with SPIO at a concentration of 25 μg/mL, nearly all cells were shown to contain iron. Interestingly, cellular apoptosis and surface expression of CD80, CD86, major histocompatibility II, and chemokine receptor 7 in mature dendritic cells were not affected to any significant extent by SPIO labeling. T cell activation was maintained at a low ratio of dendritic cells to T cells.

CONCLUSION

SPIO nanoparticles have good superparamagnetic behavior, highly biocompatible characteristics, and are suitable for use in further study of the migratory behavior and biodistribution of dendritic cells in vivo.

摘要

背景

本研究探讨了合成超顺磁性氧化铁（SPIO）对树突状细胞的影响，并为这些细胞的标记提供了一种可能的方法。

方法

制备 SPIO 纳米颗粒，并对其形态和磁性能进行了表征。将这些颗粒内吞入由小鼠骨髓生成的树突状细胞中。通过普鲁士蓝染色和定量分光光度法分析标记效率和细胞摄取。同时，通过流式细胞术和混合淋巴细胞反应实验探讨了 SPIO 标记的树突状细胞的表面分子、细胞凋亡和功能特性。

结果

合成的纳米颗粒呈球形且具有良好的超顺磁性。幼稚和成熟树突状细胞中铁的平均浓度分别为 31.8 ± 0.7 μg 和 35.6 ± 1.0 μg/1 × 10(6)细胞。在浓度为 25 μg/mL 的 SPIO 孵育 12 小时后，几乎所有细胞都含有铁。有趣的是，SPIO 标记对成熟树突状细胞的细胞凋亡和 CD80、CD86、主要组织相容性 II 和趋化因子受体 7 的表面表达没有任何显著影响。T 细胞的激活保持在树突状细胞与 T 细胞的低比例。

结论

SPIO 纳米颗粒具有良好的超顺磁性、高度的生物相容性特点，适合进一步研究树突状细胞在体内的迁移行为和生物分布。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/48c7b4688e19/ijn-6-1779f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/d9698d2b70a2/ijn-6-1779f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/7aec09206ab9/ijn-6-1779f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/e2ece20c76d5/ijn-6-1779f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/af659d440322/ijn-6-1779f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/48f47ac0a39f/ijn-6-1779f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/cf706aa28a27/ijn-6-1779f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/48c7b4688e19/ijn-6-1779f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/d9698d2b70a2/ijn-6-1779f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/7aec09206ab9/ijn-6-1779f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/e2ece20c76d5/ijn-6-1779f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/af659d440322/ijn-6-1779f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/48f47ac0a39f/ijn-6-1779f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/cf706aa28a27/ijn-6-1779f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc4c/3184937/48c7b4688e19/ijn-6-1779f7.jpg

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