Ailiani A C, Neuberger T, Brasseur J G, Banco G, Wang Y, Smith N B, Webb A G
Department of Bioengineering, Pennsylvania State University, University Park, PA, USA.
Neurogastroenterol Motil. 2014 Oct;26(10):1477-86. doi: 10.1111/nmo.12410.
Anesthetics are commonly applied in animal studies of gastrointestinal (GI) function. Different anesthetics alter smooth-muscle motility in different ways. The aim of this study is to quantify and compare non-invasively with magnetic resonance imaging (MRI) the motility patterns of the rat gut when anesthetized with inactin vs isoflurane anesthetics in the fed state.
Rats were given an oral gavage of MRI contrast agent for improved visualization of the GI tract. Two-dimensional images through the jejunum of the pre- and postanesthetized rat in the fed state were acquired every 168 ms. Image registration, segmentation, and postprocessing algorithms were applied to produce spatio-temporal maps that were used to quantify peristaltic and segmental motions in the jejunum region interspersed between periods of inactivity.
There were significantly longer periods of inactivity in the rats treated with isoflurane than in those treated with inactin (179.9 ± 22.4 s vs 17.7 ± 10.3 s). The speed of propagation and wavelength of peristalsis, and the frequency and speed of pattern switching of segmental motility, were higher (p < 0.05) in rats treated with inactin.
CONCLUSIONS & INFERENCES: Isoflurane and inactin anesthetics produce significantly different motility behavior with the rat's GI tract in the fed state. Isoflurane anesthetic, results in a reduced frequency of occurrence of motility periods and an overall reduced level of motility in comparison with inactin.
麻醉剂常用于胃肠道（GI）功能的动物研究。不同的麻醉剂以不同方式改变平滑肌运动。本研究的目的是在喂食状态下，使用磁共振成像（MRI）对用戊巴比妥钠与异氟烷麻醉的大鼠肠道运动模式进行无创量化和比较。
给大鼠经口灌胃磁共振成像造影剂以改善胃肠道的可视化。在喂食状态下，每隔168毫秒获取麻醉前后大鼠空肠的二维图像。应用图像配准、分割和后处理算法生成时空图，用于量化空肠区域在不活动期之间穿插的蠕动和节段性运动。
用异氟烷处理的大鼠不活动期明显长于用戊巴比妥钠处理的大鼠（179.9±22.4秒对17.7±10.3秒）。用戊巴比妥钠处理的大鼠蠕动的传播速度和波长以及节段性运动的模式切换频率和速度更高（p<0.05）。
异氟烷和戊巴比妥钠麻醉剂在喂食状态下使大鼠胃肠道产生明显不同的运动行为。与戊巴比妥钠相比，异氟烷麻醉导致运动期出现频率降低和整体运动水平降低。
