Tsai Jui-Chi, Lin Yi-Wen, Huang Chun-Yao, Lin Feng-Yen, Tsai Chien-Sung
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan ; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan ; Division of Cardiology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan.
Institute of Oral Biology, National Yang-Ming University, Taipei 112, Taiwan ; Division of Cardiovascular Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan.
Mediators Inflamm. 2014;2014:484510. doi: 10.1155/2014/484510. Epub 2014 Aug 31.
Human platelets express Toll-like receptors (TLR) 4. However, the mechanism by which TLR4 directly affects platelet aggregation and blood coagulation remains to be explored. Therefore, in this study, we evaluated the platelet TLR4 expression in patients who underwent CABG surgery; we explored the correlation between platelet TLR4 expression and the early outcomes in hospital of patients. Additionally, C57BL/6 and C57BL/6-Tlr(LPS-/-) mice were used to explore the roles of platelet TLR4 in coagulation by platelet aggregometry and rotation thromboelastometry. In conclusion, our results highlight the important roles of TLR4 in blood coagulation and platelet function. Of clinical relevance, we also explored novel roles for platelet TLR4 that are associated with early outcomes in cardiac surgery.
人类血小板表达Toll样受体（TLR）4。然而，TLR4直接影响血小板聚集和血液凝固的机制仍有待探索。因此，在本研究中，我们评估了接受冠状动脉旁路移植术（CABG）手术患者的血小板TLR4表达；我们探讨了血小板TLR4表达与患者住院早期结局之间的相关性。此外，使用C57BL/6和C57BL/6-Tlr（LPS-/-）小鼠通过血小板聚集测定法和旋转血栓弹力测定法来探索血小板TLR4在凝血中的作用。总之，我们的结果突出了TLR4在血液凝固和血小板功能中的重要作用。具有临床相关性的是，我们还探索了血小板TLR4与心脏手术早期结局相关的新作用。
