Ludwig Boltzmann Institute of Experimental and Clinical Traumatology, Vienna, Austria.
Thromb Res. 2012 Sep;130(3):e107-12. doi: 10.1016/j.thromres.2012.03.006. Epub 2012 Apr 4.
Interest in visco-elastic testing in different clinical scenarios has increased but few data are available on thromboelastometric findings in primates.
Blood cell count (hemoglobin, hematocrit, platelet count), coagulation parameters (prothrombin time, International Normalized Ratio, fibrinogen), and ROTEM® (Tem International GmbH, Munich, Germany) variables were analyzed using blood from 25 anesthetized male baboons and 21 non-anesthetized healthy volunteers. The platelet component of the clot was calculated as the difference in maximum clot elasticity (MCE) between the whole blood clot (EXTEM test) and the fibrin-based clot (FIBTEM test). In subgroups of each species, 10 μg abciximab was added to the regular FIBTEM reagent (cytochalasin D) for additional platelet inhibition.
Blood cell count was comparable between humans and primates. Both fibrinogen concentration (p<0.0001) and maximum clot firmness (MCF) in FIBTEM assays were significantly lower in baboons (p>0.0001, and p=0.006, respectively). PT, INR, and clotting time in NATEM assays were significantly prolonged in humans compared with baboons. MCF in NATEM, EXTEM and INTEM assays was not different between baboons and humans. Clot lysis in NATEM, EXTEM and INTEM assays was significantly higher in humans (p<0.0001). In contrast FIBTEM clot lysis was significantly higher in baboons (p=0.01). Addition of abciximab into the FIBTEM assay resulted in a significant reduction in MCF and MCE (p<0.001) and, consequently, the platelet component increased similar in both humans and baboons (p<0.001).
Activated ROTEM® tests revealed broad similarities between humans and baboons. ROTEM® assays developed for use in humans can also be used in baboons.
人们对不同临床情况下的黏弹性测试越来越感兴趣，但关于灵长类动物血栓弹性图检测结果的数据却很少。
使用来自 25 只麻醉雄性狨猴和 21 名非麻醉健康志愿者的血液，分析血细胞计数（血红蛋白、血细胞比容、血小板计数）、凝血参数（凝血酶原时间、国际标准化比值、纤维蛋白原）和 ROTEM®（Tem International GmbH，慕尼黑，德国）变量。血小板成分的凝块计算为全血凝块（EXTEM 试验）和基于纤维蛋白的凝块（FIBTEM 试验）之间最大凝块弹性（MCE）的差异。在每种物种的亚组中，将 10μg 阿昔单抗加入常规 FIBTEM 试剂（细胞松弛素 D）中，以进一步抑制血小板。
血细胞计数在人类和灵长类动物之间是可比的。FIBTEM 检测中纤维蛋白原浓度（p<0.0001）和最大凝块硬度（MCF）均显著降低（p>0.0001 和 p=0.006）。与狨猴相比，NATEM 检测中的 PT、INR 和凝血时间明显延长。NATEM、EXTEM 和 INTEM 检测中的 MCF 无明显差异狨猴和人类之间。NATEM、EXTEM 和 INTEM 检测中的凝块溶解率在人类中明显更高（p<0.0001）。相反，FIBTEM 凝块溶解率在狨猴中明显更高（p=0.01）。在 FIBTEM 检测中加入阿昔单抗可显著降低 MCF 和 MCE（p<0.001），因此血小板成分在人类和狨猴中均显著增加（p<0.001）。
激活的 ROTEM® 检测显示人类和狨猴之间存在广泛的相似性。为人类开发的 ROTEM® 检测也可用于狨猴。
