a Department of Pharmaceutics , and.
b Department of Pharmacology , Faculty of Pharmacy, Jamia Hamdard (Hamdard University) , Hamdard Nagar , New Delhi , India.
Drug Deliv. 2016 Sep;23(7):2228-2234. doi: 10.3109/10717544.2014.958625. Epub 2014 Sep 26.
The aim of the novel study was to check the efficacy of a locally applied 2%w/w nanoemulgel (NEG) of Ketoprofen (KP) in preventing the periodontitis, and was also checked NEG without KP to ensure the effect of eugenol in NEG as an oil phase.
For experimentally induced periodontitis, sterile silk ligatures (3/0) were placed around the crevices of the first left lower molar teeth of the male Wistar rats. During 8 weeks, all rats were fed with 10%w/v sucrose solution. The experimental assessment was carried out at 11 d after treatment of experimental periodontal disease (EPD) rats by various clinical parameters like gingival index (GI), tooth mobility (TM), alveolar bone loss (ABL), histological analysis, detection of TNF-α, and IL-1β in gingival tissue by ELISA and the roughness were measured by atomic force microscopy (AFM) in tapping modes.
After treatment, comparison studies with EPD were performed. NEG loaded with KP prevents significantly (p < 0.05) various parameters (GI, TM, and ABL), which were responsible for periodontitis. The histopathology of the periodontium showed that Group 3 (NEG loaded with KP) had a more significant reduction in inflammatory cell infiltration, alveolar bones resorption, and cementum (p < 0.05). In the topographical images, significant reduction in roughness of NEG loaded with KP was observed in comparison with EPD without treatment.
The study revealed the great synergistic potential of the combined NEG of an anti-inflammatory drug KP along with eugenol as the oil phase, which have potential antibacterial, analgesic, and anesthetic properties to combat periodontal disease.
本研究旨在验证局部应用 2%w/w 载酮洛芬（KP）的纳米乳凝胶（NEG）预防牙周炎的疗效，并验证不含 KP 的 NEG 以确保丁香酚作为油相在 NEG 中的作用。
对于实验性牙周炎，无菌丝线（3/0）置于雄性 Wistar 大鼠左下颌第一磨牙缝隙周围。在 8 周期间，所有大鼠均用 10%w/v 蔗糖溶液喂养。在治疗实验性牙周病（EPD）大鼠 11 天后，通过各种临床参数（如牙龈指数（GI）、牙齿松动度（TM）、牙槽骨丧失（ABL）、组织学分析、检测 TNF-α 和 IL-1β 在牙龈组织中的含量）进行实验评估，并通过原子力显微镜（AFM）在轻敲模式下测量粗糙度。
治疗后，与 EPD 进行了比较研究。载 KP 的 NEG 可显著（p<0.05）预防各种参数（GI、TM 和 ABL），这些参数与牙周炎有关。牙周组织的组织病理学显示，第 3 组（载 KP 的 NEG）炎症细胞浸润、牙槽骨吸收和牙骨质减少更为显著（p<0.05）。在形貌图像中，与未经治疗的 EPD 相比，载 KP 的 NEG 的粗糙度显著降低。
该研究揭示了载有抗炎药物 KP 的联合 NEG 与丁香酚作为油相的协同潜力，具有潜在的抗菌、镇痛和麻醉特性，可用于治疗牙周病。
