Cloning and expression of a human muscle phosphofructokinase cDNA.

作者信息

Sharma P M, Reddy G R, Vora S, Babior B M, McLachlan A

机构信息

Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, CA 92037.

出版信息

Gene. 1989 Apr 15;77(1):177-83. doi: 10.1016/0378-1119(89)90372-7.

The nucleotide sequence of a 2.86-kb cDNA clone containing the complete human muscle phosphofructokinase (PFK) protein-coding region was determined. It comprises 76 bp of 5'-untranslated sequence, 2340 bp encoding human muscle PFK polypeptide, and 399 bp of 3'-untranslated sequence plus a poly(A) tract. A retroviral vector was utilized to express the product of this coding sequence in mouse fibroblasts. The PFK-coding cDNA was shown to code for an enzymatically active polypeptide by immunoprecipitation analysis and DEAE-Sephadex A-25 chromatography.