枯草芽孢杆菌的质子动力势与运动性
Protonmotive force and motility of Bacillus subtilis.
作者信息
Shioi J I, Imae Y, Oosawa F
出版信息
J Bacteriol. 1978 Mar;133(3):1083-8. doi: 10.1128/jb.133.3.1083-1088.1978.
Abstract
Motility of Bacillus subtilis was inhibited within a few minutes by a combination of valinomycin and a high concentration of potassium ions in the medium at neutral pH. Motility was restored by lowering the concentration of valinomycin or potassium ions. The valinomycin concentration necessary for motility inhibition was determined at various concentrations of potassium ions and various pH's. At pH 7.5, valinomycin of any concentration did not inhibit the motility, when the potassium ion concentration was lower than 9 mM. In the presence of 230 mM potassium ion, the motility inhibition by valinomycin was not detected at pH lower than 6.1. These results are easily explained by the idea that the motility of B. subtilis is supported by the electrochemical potential difference of the proton across the membrane, or the protonmotive force. The electrochemical potential difference necessary for motility was estimated to be about -90 mV.
摘要
在中性pH值条件下，缬氨霉素与培养基中高浓度钾离子联合作用，几分钟内即可抑制枯草芽孢杆菌的运动性。降低缬氨霉素或钾离子浓度可恢复运动性。在不同钾离子浓度和不同pH值条件下，测定了抑制运动性所需的缬氨霉素浓度。在pH 7.5时，当钾离子浓度低于9 mM时，任何浓度的缬氨霉素均不抑制运动性。在存在230 mM钾离子的情况下，在pH低于6.1时未检测到缬氨霉素对运动性的抑制作用。这些结果很容易用以下观点来解释：枯草芽孢杆菌的运动性由质子跨膜的电化学势差或质子动力支持。运动性所需的电化学势差估计约为-90 mV。
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