Ashraf Shamaila, Brockman-Schneider Rebecca, Gern James E
Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA,
Methods Mol Biol. 2015;1221:63-70. doi: 10.1007/978-1-4939-1571-2_6.
Rhinovirus-C (RV-C) were discovered recently using molecular methods. Classical methods failed to detect them since they could not grow in standard cell culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells of human airway at air-liquid interface (ALI). These cultures supported efficient replication of RV-C strains as determined by quantitative RT-PCR. This system has enabled study of the biological characteristics of RV-C strains, including quantitative research.
鼻病毒C型(RV-C)是最近利用分子方法发现的。由于它们无法在标准细胞培养中生长,传统方法未能检测到它们。现在已有几种RV-C毒株的完整基因组序列,但关于其生物学特性的信息却很少。HRV-C最初是在器官培养中生长的,最近,我们开发了一种在气液界面(ALI)的人气道分化上皮细胞中培养RV-C毒株的系统。通过定量逆转录聚合酶链反应确定,这些培养物支持RV-C毒株的高效复制。该系统使得对RV-C毒株生物学特性的研究成为可能,包括定量研究。