Wang Hui, Kobayashi Shinichi, Hiraide Hatsue, Cui Zongjun, Mochidzuki Kazuhiro
Institute of Industrial Science, The University of Tokyo, Tokyo, 153-8505, Japan,
Appl Biochem Biotechnol. 2015 Jan;175(1):287-99. doi: 10.1007/s12010-014-1242-2. Epub 2014 Sep 28.
Nonenzymatic protein was added to cellulase hydrolysis and simultaneous saccharification and fermentation (SSF) of different biomass materials. Adding bovine serum albumin (BSA) and corn steep before cellulase enhanced enzyme activity in solution and increased cellulose and xylose conversion rates. The cellulose conversion rate of filter paper hydrolysis was increased by 32.5 % with BSA treatment. When BSA was added before cellulase, the remaining activity in the solution was higher than that in a control without BSA pretreatment. During SSF with pretreated rice straw as the substrate, adding 1.0 mg/mL BSA increased the ethanol yield by 13.6 % and final xylose yield by 42.6 %. The results indicated that lignin interaction is not the only mechanism responsible for the positive BSA effect. BSA had a stabilizing effect on cellulase and relieved cumulative sugar inhibition of enzymatic hydrolysis of biomass materials. Thus, nonenzymatic protein addition represents a promising strategy in the biorefining of lignocellulose materials.
将非酶蛋白添加到不同生物质材料的纤维素酶水解及同步糖化发酵（SSF）过程中。在纤维素酶之前添加牛血清白蛋白（BSA）和玉米浆可提高溶液中的酶活性，并提高纤维素和木糖转化率。经BSA处理后，滤纸水解的纤维素转化率提高了32.5%。当在纤维素酶之前添加BSA时，溶液中的剩余活性高于未进行BSA预处理的对照。以预处理稻草为底物进行SSF过程中，添加1.0 mg/mL BSA可使乙醇产量提高13.6%，最终木糖产量提高42.6%。结果表明，木质素相互作用并非BSA产生积极作用的唯一机制。BSA对纤维素酶具有稳定作用，并减轻了生物质材料酶水解过程中累积糖的抑制作用。因此，添加非酶蛋白是木质纤维素材料生物精炼中一种很有前景的策略。
