Institute of Industrial Science, The University of Tokyo, Tokyo 153-8505, Japan.
Institute of Industrial Science, The University of Tokyo, Tokyo 153-8505, Japan.
Bioresour Technol. 2015 Aug;190:373-80. doi: 10.1016/j.biortech.2015.04.112. Epub 2015 May 6.
Non-enzymatic proteins were added during hydrolysis of cellulose and simultaneous saccharification and fermentation (SSF) of different biomass materials. Bovine serum albumin (BSA), a model non-enzymatic protein, increased cellulose and xylose conversion efficiency and also enhanced the ethanol yield during SSF of rice straw subjected to varied pretreatments. Corn steep liquor, yeast extract, and peptone also exerted a similar effect as BSA and enhanced the enzymatic hydrolysis of rice straw. Compared to the glucose yields obtained after enzymatic hydrolysis of rice straw in the absence of additives, the glucose yields after 72h of hydrolysis increased by 12.7%, 13.5%, and 13.7% after addition of the corn steep liquor, yeast extract, and peptone, respectively. This study indicated the use of BSA as an alternative to intensive pretreatment of lignocellulosic materials for enhancing enzymatic digestibility. The utilization of non-enzymatic protein additives is promising for application in glucose and ethanol production from lignocellulosic materials.
非酶蛋白在纤维素的水解和不同生物质材料的同步糖化和发酵（SSF）过程中被添加。牛血清白蛋白（BSA）作为一种模型非酶蛋白，提高了纤维素和木糖的转化效率，并且在经过不同预处理的稻草 SSF 过程中提高了乙醇的产量。玉米浆、酵母提取物和蛋白胨也像 BSA 一样发挥了类似的作用，并增强了稻草的酶水解。与没有添加剂的情况下酶解稻草获得的葡萄糖产量相比，分别添加玉米浆、酵母提取物和蛋白胨后，水解 72 小时后的葡萄糖产量分别提高了 12.7%、13.5%和 13.7%。本研究表明，BSA 可替代木质纤维素材料的强化预处理，以提高酶解可及性。非酶蛋白添加剂的利用有望应用于从木质纤维素材料生产葡萄糖和乙醇。
