Grieve Kate, Palazzo Laurent, Dalimier Eugenie, Vielh Philippe, Fabre Monique
Institut de la Vision, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France.
Clinique du Trocadéro, Paris, France.
Gastrointest Endosc. 2015 Feb;81(2):342-50. doi: 10.1016/j.gie.2014.06.037. Epub 2014 Sep 26.
Rapid on-site evaluation of cytologic specimens is a way of determining the adequacy of fine-needle aspiration (FNA). However, alternatives may be useful when the presence of a cytotechnologist and/or pathologist is not possible.
To evaluate the feasibility of using full-field optical coherence tomography (FFOCT) for FNA specimen quality assessment.
FFOCT images were acquired on gastric, pancreatic, pelvic, and lymph-node formalin-fixed FNA specimens and were compared with histology of the same samples.
Pathology suite in a hospital.
Fourteen patients undergoing gastric, pancreatic, pelvic, or lymph-node EUS-guided FNA biopsy.
FFOCT imaging on formalin-fixed samples before histologic procedures.
FFOCT imaging feasibility and visibility of normal and abnormal features on images.
FFOCT imaging was possible. Blood, mucus, muscle, collagen, and digestive mucosa could be identified as well as abnormal architectural features including infiltrative pancreatic ductal carcinoma and a neuroendocrine neoplasm. Lesions at the individual cell level could not be detected.
The study was performed on a limited number of cases.
FFOCT offers rapid, noninvasive, nondestructive imaging of FNA biopsy specimens. In the future, it could be performed in the endoscopy suite to improve detection of satisfactory specimens and obviate the need for rapid on-site evaluation.
对细胞学标本进行快速现场评估是确定细针穿刺抽吸（FNA）标本是否充足的一种方法。然而，当细胞技术人员和/或病理学家无法到场时，其他方法可能会有用。
评估使用全场光学相干断层扫描（FFOCT）进行FNA标本质量评估的可行性。
在胃、胰腺、盆腔和淋巴结的福尔马林固定FNA标本上采集FFOCT图像，并与相同样本的组织学结果进行比较。
一家医院的病理科。
14例接受胃、胰腺、盆腔或淋巴结超声内镜引导下FNA活检的患者。
在组织学检查前对福尔马林固定样本进行FFOCT成像。
FFOCT成像的可行性以及图像上正常和异常特征的可见性。
FFOCT成像可行。可以识别出血液黏液、肌肉、胶原蛋白和消化黏膜，以及包括浸润性胰腺导管癌和神经内分泌肿瘤在内的异常结构特征。无法检测到单个细胞水平的病变。
该研究仅在有限数量的病例上进行。
FFOCT可为FNA活检标本提供快速、无创、无损的成像。未来，它可在内镜检查室进行，以提高对满意标本检测的准确性，并消除快速现场评估的必要性。
