Nelson Matthew D, Lee Kun He, Churgin Matthew A, Hill Andrew J, Van Buskirk Cheryl, Fang-Yen Christopher, Raizen David M
Department of Neurology, Perelman School of Medicine, University of Pennsylvania, 462 Stemmler Hall, 415 Curie Boulevard, Philadelphia, PA 19104, USA.
Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA.
Curr Biol. 2014 Oct 20;24(20):2406-10. doi: 10.1016/j.cub.2014.08.037. Epub 2014 Sep 25.
Among the most important decisions an animal makes is whether to engage in active movement and feeding behavior or to become quiescent. The molecular signaling mechanisms underlying this decision remain largely unknown. The nematode Caenorhabditis elegans displays sleep-like quiescence following exposures that result in cellular stress. The neurosecretory ALA neuron is required for this stress-induced recovery quiescence, but the mechanisms by which ALA induces quiescence have been unknown. We report here that quiescence induced by heat stress requires ALA depolarization and release of FMRFamide-like neuropeptides encoded by the flp-13 gene. Optogenetic activation of ALA reduces feeding and locomotion in a FLP-13-dependent manner. Overexpression of flp-13 is sufficient to induce quiescent behavior during normally active periods. We have here identified a major biological role for FMRFamide-like neuropeptides in nematodes, and we suggest that they may function in a similar capacity in other organisms.
动物做出的最重要决定之一是是否进行主动运动和摄食行为，还是进入静止状态。这一决定背后的分子信号传导机制在很大程度上仍然未知。线虫秀丽隐杆线虫在受到导致细胞应激的刺激后会表现出类似睡眠的静止状态。神经分泌性ALA神经元是这种应激诱导的恢复性静止所必需的，但ALA诱导静止的机制尚不清楚。我们在此报告，热应激诱导的静止需要ALA去极化并释放由flp-13基因编码的类FMRF酰胺神经肽。对ALA进行光遗传学激活会以FLP-13依赖的方式减少摄食和运动。flp-13的过表达足以在正常活跃期诱导静止行为。我们在此确定了类FMRF酰胺神经肽在线虫中的一个主要生物学作用，并认为它们可能在其他生物体中发挥类似的功能。
