Pawłowska Julia, Wilk Mateusz, Sliwińska-Wyrzychowska Anna, Mętrak Monika, Wrzosek Marta
Department of Systematics and Plant Geography, Faculty of Biology, The University of Warsaw, Al. Ujazdowskie 4, 00-478 Warsaw, Poland.
College of Inter-Faculty Individual Studies in Mathematics and Natural Sciences, The University of Warsaw, Żwirki i Wigury 93, 02-089 Warsaw, Poland.
Symbiosis. 2014;63(2):87-97. doi: 10.1007/s13199-014-0291-1. Epub 2014 Sep 10.
Endophytes are a large and diverse group of fungi that colonize healthy plant tissues without causing any symptoms. The majority of studies have focused on angiosperm and conifer hosts and few have examined the endophytes of lycophytes. In the present study, we characterized culturable endophytic fungi in two closely related species ( and ) from pine, beech, oak and spruce forests across Poland. More than 400 strains were isolated but only 18 Ascomycete species were identified. Members of the Dothideomycetes dominated the fungal endophyte communities in . The most abundant taxa cultured were (from ) and (from ). Five taxa were isolated exclusively from , but only two of them ( and sp.) were relatively abundant. Two taxa were only found in , namely: and an unidentified Dothideomycete. The taxon assigned as Ascomycota 2 (SH219457.06FU) was isolated only from strobili of both host species. Direct PCR and cloning from shoots revealed a substantially greater endophyte richness compared with the results from culturing.
内生菌是一类庞大且多样的真菌，它们定殖于健康植物组织中却不引发任何症状。大多数研究聚焦于被子植物和针叶树宿主，很少有研究考察石松类植物的内生菌。在本研究中，我们对来自波兰各地的松林、山毛榉林、橡树林和云杉林中两个近缘物种（[物种名称缺失]和[物种名称缺失]）的可培养内生真菌进行了特征描述。分离出了400多个菌株，但仅鉴定出18种子囊菌。座囊菌纲成员在[物种名称缺失]的真菌内生菌群落中占主导地位。培养出的最丰富类群是[类群名称缺失]（来自[宿主名称缺失]）和[类群名称缺失]（来自[宿主名称缺失]）。有5个类群仅从[物种名称缺失]中分离得到，但其中只有两个（[类群名称缺失]和[类群名称缺失]种）相对丰富。有两个类群仅在[物种名称缺失]中被发现，即：[类群名称缺失]和一种未鉴定的座囊菌。被归类为子囊菌门2（SH219457.06FU）的类群仅从两个宿主物种的球果中分离得到。与培养结果相比，对[物种名称缺失]嫩枝进行直接PCR和克隆显示内生菌丰富度显著更高。
