Tian Jin-ying, Tao Rong-ya, Zhang Xiao-lin, Liu Qian, He Yi-Bo, Su Ya-lun, Ji Teng-fei, Ye Fei
Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, 100050, China.
Phytother Res. 2015 Jan;29(1):86-92. doi: 10.1002/ptr.5230. Epub 2014 Sep 30.
Natural product Hypericum perforatum L. has been used in folk medicine to improve mental performance. However, the effect of H. perforatum L. on metabolism is still unknown. In order to test whether H. perforatum L. extract (EHP) has an effect on metabolic syndrome, we treated diet induced obese (DIO) C57BL/6J mice with the extract. The chemical characters of EHP were investigated with thin-layer chromatography, ultraviolet, high-performance liquid chromatography (HPLC), and HPLC-mass spectrometry fingerprint analysis. Oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and the glucose infusion rate (GIR) in hyperinsulinemic-euglycemic clamp test were performed to evaluate the glucose metabolism and insulin sensitivity. Skeletal muscle was examined for lipid metabolism. The results suggest that EHP can significantly improve the glucose and lipid metabolism in DIO mice. In vitro, EHP inhibited the catalytic activity of recombinant human protein tyrosine phosphatase 1B (PTP1B) and reduced the protein and mRNA levels of PTP1B in the skeletal muscle. Moreover, expressions of genes related to fatty acid uptake and oxidation were changed by EHP in the skeletal muscle. These results suggest that EHP may improve insulin resistance and lipid metabolism in DIO mice.
天然产物贯叶连翘已被用于民间医学以改善精神状态。然而，贯叶连翘对新陈代谢的影响仍不清楚。为了测试贯叶连翘提取物（EHP）是否对代谢综合征有影响，我们用该提取物处理了饮食诱导肥胖（DIO）的C57BL/6J小鼠。通过薄层色谱、紫外、高效液相色谱（HPLC）和HPLC-质谱指纹分析研究了EHP的化学特性。进行口服葡萄糖耐量试验（OGTT）、胰岛素耐量试验（ITT）以及高胰岛素-正常血糖钳夹试验中的葡萄糖输注率（GIR）以评估葡萄糖代谢和胰岛素敏感性。检测骨骼肌的脂质代谢。结果表明，EHP可显著改善DIO小鼠的葡萄糖和脂质代谢。在体外，EHP抑制重组人蛋白酪氨酸磷酸酶1B（PTP1B）的催化活性，并降低骨骼肌中PTP1B的蛋白质和mRNA水平。此外，EHP改变了骨骼肌中与脂肪酸摄取和氧化相关基因的表达。这些结果表明，EHP可能改善DIO小鼠的胰岛素抵抗和脂质代谢。
