Sarker Bapi, Singh Raminder, Silva Raquel, Roether Judith A, Kaschta Joachim, Detsch Rainer, Schubert Dirk W, Cicha Iwona, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany.
PLoS One. 2014 Sep 30;9(9):e107952. doi: 10.1371/journal.pone.0107952. eCollection 2014.
Due to the relatively poor cell-material interaction of alginate hydrogel, alginate-gelatin crosslinked (ADA-GEL) hydrogel was synthesized through covalent crosslinking of alginate di-aldehyde (ADA) with gelatin that supported cell attachment, spreading and proliferation. This study highlights the evaluation of the physico-chemical properties of synthesized ADA-GEL hydrogels of different compositions compared to alginate in the form of films. Moreover, in vitro cell-material interaction on ADA-GEL hydrogels of different compositions compared to alginate was investigated by using normal human dermal fibroblasts. Viability, attachment, spreading and proliferation of fibroblasts were significantly increased on ADA-GEL hydrogels compared to alginate. Moreover, in vitro cytocompatibility of ADA-GEL hydrogels was found to be increased with increasing gelatin content. These findings indicate that ADA-GEL hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
由于藻酸盐水凝胶的细胞-材料相互作用相对较差，通过将藻酸二醛（ADA）与支持细胞附着、铺展和增殖的明胶进行共价交联，合成了藻酸盐-明胶交联（ADA-GEL）水凝胶。本研究着重评估了与藻酸盐薄膜形式相比，不同组成的合成ADA-GEL水凝胶的物理化学性质。此外，通过使用正常人皮肤成纤维细胞，研究了与藻酸盐相比，不同组成的ADA-GEL水凝胶上的体外细胞-材料相互作用。与藻酸盐相比，成纤维细胞在ADA-GEL水凝胶上的活力、附着、铺展和增殖显著增加。此外，发现ADA-GEL水凝胶的体外细胞相容性随着明胶含量的增加而提高。这些发现表明，ADA-GEL水凝胶是一种在组织工程和再生的生物医学应用中很有前景的材料。
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