甲型和丙型流感病毒之间的嵌合核蛋白非编码区揭示了它们在翻译调控中的作用。

Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation.

作者信息

Crescenzo-Chaigne Bernadette, Barbezange Cyril, Frigard Vianney, Poulain Damien, van der Werf Sylvie

机构信息

Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France.

Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France; Université Paris Diderot Sorbonne Paris Cité, Paris, France.

出版信息

PLoS One. 2014 Sep 30;9(9):e109046. doi: 10.1371/journal.pone.0109046. eCollection 2014.

Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5' NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication.